Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Research and Development (R&D), Saeed Pathobiology & Genetics Laboratory, Tehran, Iran.
J Clin Lab Anal. 2020 Dec;34(12):e23551. doi: 10.1002/jcla.23551. Epub 2020 Sep 1.
A common problem in clinical laboratories is maintaining the stability of analytes during pre-analytical processes. The aim of this study was to systematically summarize the results of a set of studies about the biochemical analytes stability.
A literature search was performed on the Advanced search field of PubMed using the keywords: "(stability) AND (analytes OR laboratory analytes OR laboratory tests OR biochemical analytes OR biochemical tests OR biochemical laboratory tests)." A total of 56 entries were obtained. After applying the selection criteria, 20 articles were included in the study.
In the 20 included references, up to 123 different analytes were assessed. The 34 analytes in order of the most frequently studied analytes were evaluated: Alanine aminotransferase, aspartate aminotransferase, potassium, triglyceride, alkaline phosphatase, creatinine, total cholesterol, albumin, lactate dehydrogenase, sodium, calcium, γ-glutamyltransferase, total bilirubin, urea, creatine kinase, inorganic phosphate, total protein, uric acid, amylase, chloride, high-density lipoprotein, magnesium, glucose, C-reactive protein, bicarbonate, ferritin, iron, lipase, transferrin, cobalamin, cortisol, folate, free thyroxine, and thyroid-stimulating hormone. Stable test results could be varied between 2 hours and 1 week according to the type of samples and/or type of blood collection tubes on a basic classification set as refrigerated or room temperature.
Biochemical analytes stability could be improved if the best pre-analytical approaches are used.
临床实验室中一个常见的问题是在分析前过程中保持分析物的稳定性。本研究旨在系统总结一组关于生化分析物稳定性的研究结果。
在 PubMed 的高级搜索字段中使用关键词“(稳定性) AND (分析物或实验室分析物或实验室测试或生化分析物或生化测试或生化实验室测试)”进行文献检索。共获得 56 项条目。应用选择标准后，有 20 篇文章纳入研究。
在纳入的 20 篇参考文献中，评估了多达 123 种不同的分析物。评估了按最常研究分析物顺序排列的 34 种分析物：丙氨酸氨基转移酶、天冬氨酸氨基转移酶、钾、甘油三酯、碱性磷酸酶、肌酐、总胆固醇、白蛋白、乳酸脱氢酶、钠、钙、γ-谷氨酰转移酶、总胆红素、尿素、肌酸激酶、无机磷、总蛋白、尿酸、淀粉酶、氯、高密度脂蛋白、镁、葡萄糖、C 反应蛋白、碳酸氢盐、铁蛋白、铁、脂肪酶、转铁蛋白、钴胺素、皮质醇、叶酸、游离甲状腺素和促甲状腺激素。根据冷藏或室温的基本分类集，不同类型的样本和/或采血管的稳定性测试结果可以在 2 小时至 1 周之间变化。
如果使用最佳的分析前方法，可以提高生化分析物的稳定性。
