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纤维素酶和碱性处理改善猪肠道对菜籽粕难降解纤维的微生物降解作用。

Cellulase and Alkaline Treatment Improve Intestinal Microbial Degradation of Recalcitrant Fibers of Rapeseed Meal in Pigs.

作者信息

Long Cheng, Rösch Christiane, de Vries Sonja, Schols Henk, Venema Koen

机构信息

Faculty of Science and Engineering, Centre for Healthy Eating & Food Innovation, Maastricht University -Campus Venlo, 5928 RC Venlo, The Netherlands.

School of Nutrition and Translational Research in Metabolism (NUTRIM), Maastricht University, 6200 MD Maastricht, The Netherlands.

出版信息

J Agric Food Chem. 2020 Sep 30;68(39):11011-11025. doi: 10.1021/acs.jafc.0c03618. Epub 2020 Sep 16.

DOI:10.1021/acs.jafc.0c03618
PMID:32871071
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7530897/
Abstract

The aim of the current study was to investigate whether degradation of rapeseed meal (RSM) by a swine gut microbiota consortium was improved by modifying RSM by treatment with cellulase (CELL), two pectinases (PECT), or alkaline (ALK) compared to untreated RSM and to assess whether microbiota composition and activity changed. The predicted relative abundances of carbohydrate digestion and absorption, glycolysis, pentose phosphate pathway, and pyruvate metabolism were significantly increased upon CELL and ALK feeding, and CELL and ALK also exhibited increased total short-chain fatty acid (SCFA) production compared to CON. , , and were significantly positively correlated with SCFA production. Findings were validated in ileal cannulated pigs, which showed that CELL and ALK increased fiber degradation of RSM. In conclusion, CELL and ALK rather than PECT1 or PECT2 increased fiber degradation in RSM, and this information could guide feed additive strategies to improve efficiency and productivity in the swine industry.

摘要

本研究的目的是调查与未处理的菜籽粕（RSM）相比，用纤维素酶（CELL）、两种果胶酶（PECT）或碱（ALK）处理对猪肠道微生物群联合体降解RSM的效果是否有改善，并评估微生物群组成和活性是否发生变化。饲喂CELL和ALK后，碳水化合物消化吸收、糖酵解、磷酸戊糖途径和丙酮酸代谢的预测相对丰度显著增加，与对照组相比，CELL和ALK还表现出总短链脂肪酸（SCFA）产量增加。 、 和 与SCFA产量呈显著正相关。在回肠造瘘猪中验证了这些发现，结果表明CELL和ALK增加了RSM的纤维降解。总之，CELL和ALK而非PECT1或PECT2增加了RSM中的纤维降解，这些信息可为提高养猪业效率和生产力的饲料添加剂策略提供指导。

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