In Vitro Fermentation Characteristics and Fiber-Degrading Enzyme Kinetics of Cellulose, Arabinoxylan, -Glucan and Glucomannan by Pig Fecal Microbiota.

Non-digestible polysaccharides are of great significance to human and animal intestinal health. Cellulose, arabinoxylan, -glucan and glucomannan were selected in the present study to investigate the fermentation characteristics and fiber-degrading enzyme kinetics by inoculating pig fecal microbiota in vitro. Our results showed that fermentation of arabinoxylan and -glucan produced the highest amount of acetate and lactate, respectively. The abundance of was the highest in -glucan group and positively correlated with lactate and acetate. Glucomannan fermentation produced the highest amount of butyrate, and the abundance of and were the lowest. A significant negative correlation was found between , and butyrate. Exo-1,4-xylanase had the highest activity at 24 h during arabinoxylan fermentation. The activity of -glucosidase and -mannosidase at 36 h were higher than those at 15 h in the glucomannan group. The abundance of was positively correlated with -glucosidase while and were negatively correlated with -xylosidase. Our findings demonstrated the -glucan and arabinoxylan promote proliferation of , with the preference to secret -glucosidase, -mannosidase and the potential to produce lactate and acetate. Butyrate production can be improved by inhibiting the proliferation of and , which have the lack of potential to secret -xylosidase.