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兔β-珠蛋白信使核糖核酸的一种特异性二聚化

A specific dimerization of rabbit beta-globin messenger ribonucleic acid.

作者信息

Mezl V A, Hunt J A

出版信息

Biochem J. 1978 Oct 1;175(1):159-69. doi: 10.1042/bj1750159.

DOI:10.1042/bj1750159
PMID:32874
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1186050/
Abstract

Rabbit globin mRNA, when layered in low salt on 0.1 M-NaCl/sucrose gradients, separates into two peaks of material. Translation of these two RNA fractions in the wheat-germ cell-free system, hybridization against globin complementary DNA (cDNA) and cross-hybridization against cDNA species prepared from each fraction show that the first peak sedimenting at 10S is a alpha-globin mRNA and the second peak, sedimenting at approx. 15S, is beta-globin mRNA. The sedimentation rate of the beta-globin mRNA is concentration-dependent. By changing concentration and pH, it is indicated that in low-salt beta-globin mRNA adopts a conformation that leads to specific, but weak, self-dimerization during centrifugation in 0.1M-NaCl. This property permits rapid preparation of intact and relatively pure alpha- and beta-globin mRNA species.

摘要

兔珠蛋白信使核糖核酸（mRNA），当以低盐状态铺在0.1M氯化钠/蔗糖梯度上时，会分离成两个物质峰。在小麦胚芽无细胞系统中对这两个RNA组分进行翻译、与珠蛋白互补DNA（cDNA）杂交以及与从每个组分制备的cDNA物种进行交叉杂交，结果表明，第一个在10S沉降的峰是α-珠蛋白mRNA，第二个在约15S沉降的峰是β-珠蛋白mRNA。β-珠蛋白mRNA的沉降速率取决于浓度。通过改变浓度和pH值表明，在低盐条件下，β-珠蛋白mRNA会形成一种构象，导致在0.1M氯化钠中离心时发生特异性但较弱的自我二聚化。这一特性使得能够快速制备完整且相对纯净的α-和β-珠蛋白mRNA物种。

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本文引用的文献

1
THE DISSOCIATION OF HEMOGLOBIN BY INORGANIC SALTS.血红蛋白被无机盐解离
Biochemistry. 1964 Mar;3:291-6. doi: 10.1021/bi00891a002.
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Characterization of a highly efficient protein synthesizing system derived from commercial wheat germ.源自市售小麦胚芽的高效蛋白质合成系统的特性分析
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The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.用聚丙烯酰胺凝胶电泳法分离高分子量核糖核酸
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Proc Natl Acad Sci U S A. 1971 Nov;68(11):2716-9. doi: 10.1073/pnas.68.11.2716.
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Properties of mouse globin messenger ribonucleic acid and its preparation in milligram quantities.小鼠珠蛋白信使核糖核酸的特性及其毫克量的制备
Biochemistry. 1971 Aug 3;10(16):3014-21. doi: 10.1021/bi00792a005.
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A procedure for the isolation of mammalian messenger ribonucleic acid.一种分离哺乳动物信使核糖核酸的方法。
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Estimation of secondary structure in ribonucleic acids.核糖核酸二级结构的估算
Nature. 1971 Apr 9;230(5293):362-7. doi: 10.1038/230362a0.
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Three abundance classes in HeLa cell messenger RNA.HeLa细胞信使核糖核酸中的三种丰度类别。
Nature. 1974 Jul 19;250(463):199-204. doi: 10.1038/250199a0.
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The separation and identification of alpha- and beta-globin messenger ribonucleic acids.α和β珠蛋白信使核糖核酸的分离与鉴定。
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FEBS Lett. 1973 Mar 15;30(3):301-4. doi: 10.1016/0014-5793(73)80674-x.

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