Faculty of Medicine, Institute of Biomedical Sciences, University of Maribor, Taborska ulica 8, 2000, Maribor, Slovenia.
AMEU-ECM Maribor, Slovenska 17, 2000, Maribor, Slovenia.
Mol Biol Rep. 2020 Sep;47(9):6929-6940. doi: 10.1007/s11033-020-05751-6. Epub 2020 Sep 2.
Macrophages are among the most important cells of the immune system. Among other functions, they take part in almost all defense actions against foreign bodies and bacteria, being particularly important in infections, wound healing, and foreign body reactions. Considering their importance for the health of the human body, as well as their important role in several diseases, the in vitro studies based on these cells, are a crucial research field. Taking all mentioned into account, this study describes a simple isolation method of human macrophages (MFUM-HMP-001 and MFUM-HMP-002 cell lines) from peripheral blood. For this purpose, the morphology, the viability, and the phagocytotic activity of the isolated cells were tested. The Immunostaining of MFUM-HMP-001 and MFUM-HMP-002 cells confirmed the macrophage cell markers CD68, CD80, and CD163/M130. The phagocytotic activity was marked in both MFUM-HMP-001 and MFUM-HMP-002 cells, as was the phagocytosis of the pHrodo green Escherichia coli bioparticles conjugates, which was enhanced with the addition of lipopolysaccharide. The cells were stable and exhibited good growth. According to our results, both cell lines are useful for the development of novel macrophage cell-based in vitro models.
巨噬细胞是免疫系统中最重要的细胞之一。除其他功能外，它们参与了几乎所有针对异物和细菌的防御反应，在感染、伤口愈合和异物反应中尤为重要。鉴于它们对人体健康的重要性，以及它们在几种疾病中的重要作用，基于这些细胞的体外研究是一个至关重要的研究领域。考虑到所有这些因素，本研究描述了一种从外周血中分离人巨噬细胞（MFUM-HMP-001 和 MFUM-HMP-002 细胞系）的简单方法。为此，测试了分离细胞的形态、活力和吞噬活性。MFUM-HMP-001 和 MFUM-HMP-002 细胞的免疫染色证实了巨噬细胞标志物 CD68、CD80 和 CD163/M130。在 MFUM-HMP-001 和 MFUM-HMP-002 细胞中均观察到吞噬活性，并且吞噬了 pHrodo 绿色大肠杆菌生物颗粒缀合物，添加脂多糖后吞噬活性增强。细胞稳定且生长良好。根据我们的结果，这两种细胞系均可用于开发新型基于巨噬细胞的体外模型。
