School of Biological Sciences, Nanyang Technological University , Singapore, Singapore.
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University , Wuhan, China.
Plant Signal Behav. 2020 Nov 1;15(11):1812878. doi: 10.1080/15592324.2020.1812878. Epub 2020 Sep 3.
The plant-specific TCP transcription factors play pivotal roles in various processes of plant growth and development. However, little is known regarding the functions of TCPs in plant oil biosynthesis. Our recent work showed that TCP4 mediates oil production via interaction with WRINKLED1 (WRI1), an essential transcription factor governing plant fatty acid biosynthesis. WRI1 (AtWRI1) physically interacts with multiple TCPs, including TCP4, TCP10, and TCP24. Transient co-expression of with , but not or , represses oil accumulation in leaves. Increased TCP4 in transgenic plants overexpressing a miR319-resistant () decreased the expression of AtWRI1 target genes. The knockout mutant, the mutant with significant reduction of expression, and a triple mutant, display increased seed oil contents compared to the wild-type . The APETALA2 (AP2) transcription factor WRI1 is characterized by regulating fatty acid biosynthesis through cross-family interactions with multiple transcriptional, post-transcriptional, and post-translational regulators. The interacting regulator modules control the range of AtWRI1 transcriptional activity, allowing spatiotemporal modulation of lipid production. Interaction of TCP4 with AtWRI1, which results in a reduction of AtWRI1 activity, represents a newly discovered mechanism that enables the fine-tuning of plant oil biosynthesis.
植物特有的 TCP 转录因子在植物生长和发育的各个过程中发挥着关键作用。然而，关于 TCP 在植物油脂生物合成中的功能知之甚少。我们最近的工作表明，TCP4 通过与 WRINKLED1（WRI1）相互作用来介导油脂产生，WRI1 是一个控制植物脂肪酸生物合成的基本转录因子。WRI1（AtWRI1）与多个 TCP 相互作用，包括 TCP4、TCP10 和 TCP24。与 瞬时共表达，但不是 或 ，抑制 叶片中的油脂积累。在过量表达抗 miR319 的 （）的转基因植物中增加 TCP4 会降低 AtWRI1 靶基因的表达。 敲除突变体、 表达显著降低的突变体和 三重突变体与野生型相比，种子油含量增加。APETALA2（AP2）转录因子 WRI1 的特征在于通过与多个转录、转录后和翻译后调节因子的跨家族相互作用来调节脂肪酸生物合成。相互作用的调节模块控制 AtWRI1 转录活性的范围，允许脂质产生的时空调节。TCP4 与 AtWRI1 的相互作用导致 AtWRI1 活性降低，代表了一种新发现的机制，可实现植物油脂生物合成的精细调控。
