Key Laboratory of Cell Engineering in Guizhou Province, Affiliated Hospital of Zunyi Medical University, Zunyi, China.
Center of Forensic Expertise, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China.
Mol Genet Genomic Med. 2020 Nov;8(11):e1473. doi: 10.1002/mgg3.1473. Epub 2020 Sep 2.
X-chromosome short tandem repeats (X-STRs) with unique sex-linkage inheritance models play a complementary role in forensic science. Guizhou is a multiethnic province located in southwest China and some genetic evidence focusing on X-STRs for various minorities was reported. However, population data of Guizhou Tujia are scarce.
A total of 507 Guizhou Tujia individuals were profiled using the AGCU X-19 STR kit. Allele frequencies and forensic parameters were calculated. Additionally, population genetic relationships between Guizhou Tujia and other 19 populations were explored.
A total of 257 alleles with the allele frequencies ranged from 0.0013 to 0.6098 were found. The combined power of discrimination in males and females and mean exclusion chances in all case scenarios were all greater than 0.99999. Population comparisons showed Guizhou Tujia had a homogeneity with all Han populations from different administrative regions, and other ethnic populations residing in Guizhou, while had obviously genetic heterogeneity with the Altaic family populations except Xibe.
Nineteen X-STRs can afford a reliable and informative database of Guizhou Tujia population for human identification and paternity testing, especially in complex biological relations. The genetic relationships of Chinese are significantly influenced by the geographic position and ethnolinguistic origin.
具有独特性连锁遗传模式的 X 染色体短串联重复序列（X-STRs）在法医学中起着补充作用。贵州是中国西南部的一个多民族省份，已有一些针对不同少数民族的 X-STR 遗传证据的报道。然而，贵州土家族的群体数据却很少。
本研究使用 AGCU X-19 STR 试剂盒对 507 名贵州土家族个体进行了基因分型。计算了等位基因频率和法医学参数。此外，还探讨了贵州土家族与其他 19 个人群之间的群体遗传关系。
共发现 257 个等位基因，其等位基因频率范围为 0.0013 至 0.6098。在所有案例情景下，男性和女性的联合鉴别力和平均排除概率均大于 0.99999。群体比较显示，贵州土家族与来自不同行政区域的所有汉族人群以及居住在贵州的其他少数民族人群具有同质性，而与阿尔泰语系人群（除了锡伯族）除了具有明显的遗传异质性。
19 个 X-STR 可提供可靠且信息丰富的贵州土家族人群人类识别和亲子关系鉴定数据库，尤其是在复杂的生物学关系中。中国人群的遗传关系受地理位置和语言民族起源的显著影响。
