Key Laboratory of Cell Engineering of Guizhou Province, Clinical Stem Cell Research Institute, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, P.R. China.
Collaborative Innovation Center of Chinese Ministry of Education, Zunyi Medical University, Zunyi, Guizhou, P.R. China.
Ann Hum Biol. 2023 Feb;50(1):345-350. doi: 10.1080/03014460.2023.2224971.
Short tandem repeats (STR) are highly polymorphic DNA markers utilised in forensic personal identification and human population genetic research. Guizhou Tujia is one of the ancient minority groups in southwest China, however, the population has not been studied using the highly discriminating 23 STR Huaxia Platinum Kit.
To obtain genetic data from 23 autosomal STRs in Guizhou Tujia and examine the population's relationship with others.
A total of 480 individuals from the Guizhou Tujia population were analysed using 23 STR loci of Huaxia Platinum Kit. Allele frequencies and forensic parameters were estimated. Population genetic relationships were calculated by Nei's genetic distances and visualised using a variety of biostatistical methods.
A total of 264 alleles were found, with allelic frequencies ranging from 0.0010 to 0.5104. The combined discrimination power (CDP) and the combined probability of paternity (CPE) of 23 STR loci were 0.9999999999999999999999999996 and 0.999999999710422, respectively. Guizhou Tujia showed closer genetic relationships with Hubei Tujia, Guizhou Gelao, and Guizhou Miao than with other populations.
We first obtained the population genetic data of Guizhou Tujia using the 23 STR system and demonstrated its value in forensic applications. Comprehensive population comparisons showed an evident genetic affinity pattern between populations that are geographically, ethnically and linguistically related.
短串联重复序列（STR）是高度多态性的 DNA 标记，用于法医个体识别和人类群体遗传研究。贵州土家族是中国西南地区的古老少数民族之一，但尚未使用高度区分的 23 个 STR 华夏白金试剂盒对其人口进行研究。
从贵州土家族的 23 个常染色体 STR 中获取遗传数据，并研究其与其他人群的关系。
使用华夏白金试剂盒的 23 个 STR 位点对 480 名贵州土家族个体进行分析。估计等位基因频率和法医参数。通过 Nei 遗传距离计算群体遗传关系，并使用多种生物统计学方法进行可视化。
共发现 264 个等位基因，等位基因频率范围为 0.0010 至 0.5104。23 个 STR 位点的综合鉴别力（CDP）和联合父权概率（CPE）分别为 0.999999999999999999999999996 和 0.999999999710422。贵州土家族与湖北土家族、贵州仡佬族和贵州苗族的遗传关系较近，与其他人群的遗传关系较远。
我们首次使用 23 个 STR 系统获得了贵州土家族的群体遗传数据，并证明了其在法医学应用中的价值。综合人群比较显示，地理、民族和语言上相关的人群之间存在明显的遗传亲和模式。
