School of Nutrition Sciences, Faculty of Health Sciences, University of Ottawa, Ottawa, ON K1N 6N5, Canada.
Department of Microbiology and Immunology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
Nutrients. 2020 Sep 1;12(9):2669. doi: 10.3390/nu12092669.
Behavior and mood disorders have been linked to gut microbiota dysbiosis through the "microbiota-gut-brain axis". Microbiota-targeting interventions are promising therapeutic modalities to restore or even maintain normal microbiome composition and activity in these disorders. Here, we test the impact of a commercial synbiotic formulation on gut microbiota composition and metabolic activity. We employed an ex-vivo continuous fermentation model that simulates the proximal colon to assess the effect of this formulation on microbiota structure and functionality as compared to no treatment control and microcrystalline cellulose as a dietary fiber control. The test formulation did not alter the diversity of gut microbiota over 48 h of treatment. However, it induced the enrichment of , and . The test formulation significantly increased the level of microbiota-generated butyrate within 12 h of treatment as compared to 24 h required by microcrystalline cellulose to boost its production. The test formulation did not lead to a significant change in amino acid profiles. These results provide evidence of potential benefits related to synbiotic effects and general gut health and support the potential of this food formulation as a therapeutic dietary intervention in mood and behavior disorders.
行为和情绪障碍通过“微生物群-肠-脑轴”与肠道微生物失调有关。针对微生物群的干预措施是有希望的治疗方式，可以恢复甚至维持这些疾病中正常的微生物组组成和活性。在这里，我们测试了一种商业共生元配方对肠道微生物群落组成和代谢活性的影响。我们采用了一种模拟近端结肠的体外连续发酵模型，以评估与无治疗对照和微晶纤维素作为膳食纤维对照相比，该配方对微生物群落结构和功能的影响。该测试配方在 48 小时的治疗过程中并未改变肠道微生物群落的多样性。然而，它诱导了 的富集。与微晶纤维素需要 24 小时才能促进其产生相比，该测试配方在 12 小时的治疗后显著增加了微生物群产生的丁酸盐水平。该测试配方未导致氨基酸谱发生显著变化。这些结果为与共生元效应和一般肠道健康相关的潜在益处提供了证据，并支持这种食品配方作为情绪和行为障碍的治疗性饮食干预的潜力。
