Rasheed Farhan, Saeed Muhammad, Alikhan Nabil-Fareed, Baker David, Khurshid Mohsin, Ainsworth Emma V, Turner A Keith, Imran Ambereen Anwar, Rasool Muhammad Hidayat, Saqalein Muhammad, Nisar Muhammad Atif, Fayyaz Ur Rehman Muhammad, Wain John, Yasir Muhammad, Langridge Gemma C, Ikram Aamer
Allama Iqbal Medical College & Jinnah Hospital (AIMC&JHL), Lahore 54000, Pakistan.
Department of Microbiology, Government College University, Faisalabad 38000, Pakistan.
Microorganisms. 2020 Sep 1;8(9):1336. doi: 10.3390/microorganisms8091336.
Extensively drug-resistant (XDR) Typhi has been reported in Sindh province of Pakistan since 2016. The potential for further spread is of serious concern as remaining treatment options are severely limited. We report the phenotypic and genotypic characterization of 27 XDR . Typhi isolated from patients attending Jinnah Hospital, Lahore, Pakistan. Isolates were identified by biochemical profiling; antimicrobial susceptibility was determined by a modified Kirby-Bauer method. These findings were confirmed using Illumina whole genome nucleotide sequence data. All sequences were compared to the outbreak strain from Southern Pakistan and typed using the . Typhi genotyping scheme. All isolates were confirmed by a sequence analysis to harbor an IncY plasmid and the CTX-M-15 ceftriaxone resistance determinant. All isolates were of the same genotypic background as the outbreak strain from Sindh province. We report the first emergence of XDR . Typhi in Punjab province of Pakistan confirmed by whole genome sequencing.
自2016年以来，巴基斯坦信德省已报告出现广泛耐药（XDR）伤寒杆菌。鉴于剩余的治疗选择极为有限，其进一步传播的可能性令人严重担忧。我们报告了从巴基斯坦拉合尔真纳医院就诊患者中分离出的27株XDR伤寒杆菌的表型和基因型特征。通过生化分析鉴定分离株；采用改良的 Kirby-Bauer 方法测定抗菌药物敏感性。使用Illumina全基因组核苷酸序列数据证实了这些发现。将所有序列与来自巴基斯坦南部的暴发菌株进行比较，并使用伤寒杆菌基因分型方案进行分型。通过序列分析确认所有分离株均携带IncY质粒和CTX-M-15头孢曲松耐药决定簇。所有分离株与信德省暴发菌株具有相同的基因型背景。我们报告了通过全基因组测序确认在巴基斯坦旁遮普省首次出现XDR伤寒杆菌。
