State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.
Genome Biol. 2020 Sep 3;21(1):230. doi: 10.1186/s13059-020-02146-5.
Fine-tuning quantitative traits for continuous subtle phenotypes is highly advantageous. We engineer the highly conserved upstream open reading frame (uORF) of FvebZIPs1.1 in strawberry (Fragaria vesca), using base editor A3A-PBE. Seven novel alleles are generated. Sugar content of the homozygous T1 mutant lines is 33.9-83.6% higher than that of the wild-type. We also recover a series of transgene-free mutants with 35 novel genotypes containing a continuum of sugar content. All the novel genotypes could be immediately fixed in subsequent generations by asexual reproduction. Genome editing coupled with asexual reproduction offers tremendous opportunities for quantitative trait improvement.
微调连续微妙表型的数量性状具有很大的优势。我们利用碱基编辑器 A3A-PBE 对草莓（ Fragaria vesca ）中高度保守的上游开放阅读框（uORF）FvebZIPs1.1 进行了工程改造。生成了七个新的等位基因。纯合 T1 突变体系的糖含量比野生型高 33.9-83.6%。我们还恢复了一系列不含转基因的突变体，它们含有 35 种具有连续糖含量的新基因型。所有新的基因型都可以通过无性繁殖立即在后续世代中固定下来。基因组编辑与无性繁殖相结合为数量性状的改良提供了巨大的机会。
