State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.
Nat Biotechnol. 2020 May;38(5):582-585. doi: 10.1038/s41587-020-0455-x. Epub 2020 Mar 16.
Prime editors, which are CRISPR-Cas9 nickase (H840A)-reverse transcriptase fusions programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells without donor DNA or double-strand breaks. We adapted prime editors for use in plants through codon, promoter, and editing-condition optimization. The resulting suite of plant prime editors enable point mutations, insertions and deletions in rice and wheat protoplasts. Regenerated prime-edited rice plants were obtained at frequencies of up to 21.8%.
先导编辑体是一种 CRISPR-Cas9 核酸酶（H840A）-逆转录酶融合蛋白，与先导编辑指导 RNA（pegRNA）共同作用，可以在哺乳动物细胞中不依赖供体 DNA 或双链断裂的情况下编辑碱基。我们通过密码子、启动子和编辑条件的优化，使先导编辑体适应于植物。由此产生的一套植物先导编辑体可以在水稻和小麦原生质体中实现点突变、插入和缺失。高达 21.8%的频率获得了经先导编辑的再生水稻植株。
