Almeida-Paes Rodrigo, Almeida Marcos Abreu, Baeza Lilian Cristiane, Marmello Leticia Andrade Mendes, Trugilho Monique Ramos de Oliveira, Nosanchuk Joshua Daniel, Soares Celia Maria de Almeida, Valente Richard Hemmi, Zancopé-Oliveira Rosely Maria
Mycology Laboratory, Evandro Chagas National Institute of Infectious Diseases, Fiocruz, Rio de Janeiro 21040-900, Brazil.
Center for Medical and Pharmaceutical Sciences, State University of Western Paraná, Cascavel, Paraná 85819-110, Brazil.
J Fungi (Basel). 2020 Dec 1;6(4):328. doi: 10.3390/jof6040328.
Species of the genus cause a systemic infection in human patients. Yeast cells of spp. produce melanin in the presence of L-dihydroxyphenylalanine and during infection, which may impact the pathogen's survival in the host. To better understand the metabolic changes that occur in melanized spp. cells, a proteomic approach was performed to compare melanized and non-melanized and yeast cells. Melanization was induced using L-dihydroxyphenylalanine as a precursor, and quantitative proteomics were performed using reversed-phase nano-chromatography coupled to high-resolution mass spectrometry. When comparing melanized versus non-melanized cells, 1006 and 582 differentially abundant/detected proteins were identified for and , respectively. Functional enrichment and comparative analysis revealed 30 important KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways in melanized and 18 in , while differentially abundant proteins from non-melanized cells from these species were involved in 21 and 25 enriched pathways, respectively. Melanized cells presented an abundance of additional virulence-associated proteins, such as phospholipase, proteases, superoxide dis-mutases, heat-shock proteins, adhesins, and proteins related to vesicular transport. The results suggest that L-dihydroxyphenylalanine increases the virulence of spp. through complex mechanisms involving not only melanin but other virulence factors as well.
该属的物种会在人类患者中引发全身感染。某些种的酵母细胞在L - 二羟基苯丙氨酸存在的情况下以及感染期间会产生黑色素，这可能会影响病原体在宿主体内的存活。为了更好地了解黑色素化的某些种细胞中发生的代谢变化，采用蛋白质组学方法比较黑色素化和未黑色素化的某些种酵母细胞。使用L - 二羟基苯丙氨酸作为前体诱导黑色素化，并使用反相纳米色谱与高分辨率质谱联用进行定量蛋白质组学分析。在比较黑色素化细胞与未黑色素化细胞时，分别为某些种和另一些种鉴定出1006个和582个差异丰富/检测到的蛋白质。功能富集和比较分析显示，黑色素化的某些种中有30条重要的KEGG（京都基因与基因组百科全书）途径，另一些种中有18条，而来自这些物种未黑色素化细胞的差异丰富蛋白质分别参与了21条和25条富集途径。黑色素化细胞呈现出大量额外的毒力相关蛋白，如磷脂酶、蛋白酶、超氧化物歧化酶、热休克蛋白、黏附素以及与囊泡运输相关的蛋白。结果表明，L - 二羟基苯丙氨酸通过不仅涉及黑色素而且还涉及其他毒力因子的复杂机制增加了某些种的毒力。
