Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Bākhtarān, Iran.
Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Bākhtarān, Iran.
Sci Rep. 2020 Sep 3;10(1):14511. doi: 10.1038/s41598-020-71365-4.
In this study, a simple technique was developed for the electrochemical detection of anionic analytes in weakly supported media. This was conducted by the use of electrochemical paper-based analytical devices (ePADs). A sensing platform was modified with nereistoxin and used to determine nitrite as a case study. The electrochemical response was improved due to the accelerated electron transfer between the sensing platform and the nitrite through the electrostatic interaction of the amino group of nereistoxin and the nitrite. The electrocatalytic current of the nitrite in the presence of nereistoxin was enhanced in the weakly supported media. By using nereistoxin as a signal enhancer, 97% of the electrochemical signal was obtained at the low ionic strength of the electrolyte, while less than 35% of this signal was obtained in the absence of nereistoxin. The limit of detection was as low as 20 nM using an ePAD. Generally, the proposed ePAD serves as a promising, efficient and low-cost device for sensing applications in weakly supported media.
在这项研究中，开发了一种简单的技术，用于在弱支持介质中电化学检测阴离子分析物。这是通过使用基于纸张的电化学分析器件（ePAD）来实现的。传感平台用神经毒素进行修饰，并用作亚硝酸盐的案例研究来确定其含量。由于通过神经毒素的氨基与亚硝酸盐之间的静电相互作用，传感平台与亚硝酸盐之间的电子转移得到加速，因此电化学响应得到了改善。在弱支持介质中，存在神经毒素时亚硝酸盐的电催化电流得到增强。通过使用神经毒素作为信号增强剂，在电解质的低离子强度下获得了 97%的电化学信号，而在没有神经毒素的情况下，仅获得了不到 35%的信号。使用 ePAD 的检出限低至 20 nM。总的来说，所提出的 ePAD 作为一种在弱支持介质中用于传感应用的有前途、高效且低成本的器件。
