The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, PR China.
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, PR China.
Biosens Bioelectron. 2017 Dec 15;98:478-485. doi: 10.1016/j.bios.2017.06.061. Epub 2017 Jul 1.
Paper-based microfluidic biosensors have recently attracted increasing attentions in point-of-care testing (POCT) territories benefiting from their affordable, accessible and eco-friendly features, where technologies for fabricating such biosensors are preferred to be equipment free, easy-to-operate and capable of rapid prototyping. In this work, we developed a pen-on-paper (PoP) strategy based on two custom-made pens, i.e., a wax pen and a conductive-ink pen, to fully write paper-based microfluidic biosensors through directly writing both microfluidic channels and electrodes. Particularly, the proposed wax pen is competent to realize one-step fabrication of wax channels on paper, as the melted wax penetrates into paper during writing process without any post-treatments. The practical applications of the fabricated paper-based microfluidic biosensors are demonstrated by both colorimetric detection of Salmonella typhimurium DNA with detection limit of 1nM and electrochemical measurement of glucose with detection limit of 1mM. The developed PoP strategy for making microfluidic biosensors on paper characterized by true simplicity, prominent portability and excellent capability for rapid prototyping shows promising prospect in POCT applications.
基于纸的微流控生物传感器最近在即时检测（POCT）领域引起了越来越多的关注，其具有经济实惠、易于获取和环保的特点，因此，制造这种生物传感器的技术更倾向于无需设备、易于操作且能够快速原型制作。在这项工作中，我们开发了一种基于两支定制笔的笔上纸（PoP）策略，即一支蜡笔和一支导电油墨笔，通过直接书写微流控通道和电极来完全在纸上书写基于纸的微流控生物传感器。特别是，所提出的蜡笔能够在纸上实现一步法制造蜡通道，因为在书写过程中，融化的蜡会渗透到纸中，而无需任何后处理。通过对伤寒沙门氏菌 DNA 的显色检测（检测限为 1nM）和对葡萄糖的电化学测量（检测限为 1mM），展示了所制造的基于纸的微流控生物传感器的实际应用。该 PoP 策略用于在纸上制作微流控生物传感器，其特点是真正的简单、出色的便携性和快速原型制作的出色能力，在 POCT 应用中具有广阔的前景。
