Emmert Hila, Baurecht Hansjörg, Thielking Frederieke, Stölzl Dora, Rodriguez Elke, Harder Inken, Proksch Ehrhardt, Weidinger Stephan
Department of Dermatology, Allergology and Venereology, University Hospital Schleswig-Holstein, Kiel, Germany.
Department of Epidemiology and Preventive Medicine, University of Regensburg, Regensburg, Germany.
Exp Dermatol. 2021 Oct;30(10):1398-1408. doi: 10.1111/exd.14185. Epub 2020 Sep 17.
Atopic dermatitis (AD) is driven by the interplay between a dysfunctional epidermal barrier and a skewed cutaneous immune dysregulation. As part of the complex skin barrier dysfunction, abnormalities in lipid organization and microbiome composition have been described. We set out to systematically investigate the composition of the stratum corneum lipidome, skin microbiome and skin physiology parameters at three different body sites in patients with AD and healthy volunteers.
We analysed tape strips from different body areas obtained from 10 adults with AD and 10 healthy volunteers matched for FLG mutation status for 361 skin lipid species using the Metabolon mass spectrometry platform. 16S rRNA data were available from all probands.
Our study showed that the lipid composition differs significantly between body sites and between AD patients and healthy individuals. Ceramide species NS was significantly higher in AD patients compared to healthy volunteers and was also higher in AD patients with a FLG mutation compared to AD patients without a FLG mutation. The correlation analysis of skin lipid alterations with the microbiome showed that Staphylococcus colonization in AD is positively correlated with ceramide subspecies AS, ADS, NS and NDS.
This is the first study to reveal site-specific lipid alterations and correlations with the skin microbiome in AD.
特应性皮炎（AD）是由功能失调的表皮屏障和皮肤免疫调节失衡之间的相互作用所驱动。作为复杂皮肤屏障功能障碍的一部分，脂质组织和微生物群组成异常已有相关描述。我们着手系统地研究AD患者和健康志愿者三个不同身体部位的角质层脂质组、皮肤微生物群和皮肤生理参数的组成。
我们使用Metabolon质谱平台分析了从10名患有AD的成年人和10名与FLG突变状态匹配的健康志愿者的不同身体部位获取的胶带条，检测了361种皮肤脂质种类。所有先证者均有16S rRNA数据。
我们的研究表明，身体部位之间以及AD患者与健康个体之间的脂质组成存在显著差异。与健康志愿者相比，AD患者中的神经酰胺种类NS显著更高，并且与无FLG突变的AD患者相比，有FLG突变的AD患者中该神经酰胺种类也更高。皮肤脂质改变与微生物群的相关性分析表明，AD中的葡萄球菌定植与神经酰胺亚类AS、ADS、NS和NDS呈正相关。
这是第一项揭示AD中特定部位脂质改变及其与皮肤微生物群相关性的研究。
