Karagöz Girişgen Derya, Zeynep Atay Naz, Yalçin Özge Ceren, Öztürk Elif Mey
Department of Chemistry, Institute for Graduate Science and Engineering, Boğaziçi University, Istanbul 34342, Turkey.
Department of Chemistry, Faculty of Arts and Sciences, Boğaziçi University, Istanbul 34342, Turkey.
ACS Omega. 2024 Aug 29;9(36):38044-38053. doi: 10.1021/acsomega.4c04992. eCollection 2024 Sep 10.
Spontaneous self-assembly of phospholipids into lipid vesicles in aqueous media is called liposomes, and these structures are widely used as nanocarriers in the cosmeceutical industry. Transethosomes are ethanol and edge activator-containing liposomes that are proven to be very effective in topical applications for penetrating the skin barrier. Many cosmeceutical products contain formulations with ceramides to restore the skin barrier and treat eczema. However, due to the low solubility and penetration ability of the ceramides, the effectiveness of these products is limited. In this study, a transethosome formulation containing ceramide 3 (Cer 3) was achieved by introducing varying concentrations of cholesterol and an edge activator (Tween 80) to improve the effect of the skin products used to treat eczema. The obtained transethosomes were examined in terms of size, homogeneity, zeta potential, morphology, and one-month stability. Loading capability experiments were carried out with lipophilic Chromabright and hydrophilic Ambora extract. The effect of Cer 3 on the loading of the selected payloads was evaluated. Data were analyzed statistically with linear regression analysis and two-way analysis of variance. The results showed that the inclusion of Cer 3 had almost no effect on the physical properties of the loaded or empty transethosomes. Independently of the presence of Cer 3, loading of the lipophilic compound was more efficient than that of the hydrophilic one.
磷脂在水介质中自发自组装成脂质体，这些结构在药妆行业中被广泛用作纳米载体。转质体是含有乙醇和边缘激活剂的脂质体，已被证明在穿透皮肤屏障的局部应用中非常有效。许多药妆产品都含有含神经酰胺的配方，以修复皮肤屏障和治疗湿疹。然而，由于神经酰胺的低溶解度和渗透能力，这些产品的有效性受到限制。在本研究中，通过引入不同浓度的胆固醇和边缘激活剂（吐温80），制备了含有神经酰胺3（Cer 3）的转质体配方，以提高用于治疗湿疹的皮肤产品的效果。对获得的转质体进行了大小、均匀性、zeta电位、形态和一个月稳定性方面的检测。使用亲脂性Chromabright和亲水性Ambora提取物进行了负载能力实验。评估了Cer 3对所选payload负载的影响。数据采用线性回归分析和双向方差分析进行统计分析。结果表明，加入Cer 3对负载或空转质体的物理性质几乎没有影响。无论Cer 3是否存在，亲脂性化合物的负载都比亲水性化合物更有效。
