Nunes Estéfane da Cruz, Canuto Gisele André Baptista
Departamento de Química Analítica, Instituto de Química, Universidade Federal da Bahia, Salvador, BA, Brazil.
Electrophoresis. 2020 Dec;41(24):2102-2113. doi: 10.1002/elps.202000133. Epub 2020 Sep 17.
Arboviruses, such as chikungunya, dengue, yellow fever, and zika, caused by the bite of the Aedes aegypti mosquito, have been a frequent public health problem, with a high incidence of outbreaks in tropical and subtropical countries. These diseases are easily confused with a flu-like illness and present very similar symptoms, difficult to distinguish, and treat appropriately. The effects that these infections cause in the organism are fundamentally derived from complex metabolic processes. A prominent area of science that investigates the changes in the metabolism of complex organisms is the metabolomics. Metabolomics measures the metabolites produced or altered in biological organisms, through the use of robust analytical platforms, such as separation techniques hyphenated with mass spectrometry, combined with bioinformatics. This review article presents an overview of the basic concepts of metabolomics workflow and advances in this field, and compiles research articles that use this omic approach to study these arboviruses. In this context, the metabolomics is applied to search new therapies, understand the viral replication mechanisms, and access the host-virus interactions.
由埃及伊蚊叮咬引起的虫媒病毒，如基孔肯雅病毒、登革热病毒、黄热病病毒和寨卡病毒，一直是常见的公共卫生问题，在热带和亚热带国家爆发的发病率很高。这些疾病很容易与流感样疾病混淆，症状非常相似，难以区分和进行适当治疗。这些感染在生物体中引起的影响基本上源于复杂的代谢过程。代谢组学是一门研究复杂生物体代谢变化的重要科学领域。代谢组学通过使用强大的分析平台，如与质谱联用的分离技术，并结合生物信息学，来测量生物体内产生或改变的代谢物。这篇综述文章概述了代谢组学工作流程的基本概念以及该领域的进展，并汇编了使用这种组学方法研究这些虫媒病毒的研究文章。在此背景下，代谢组学被应用于寻找新疗法、理解病毒复制机制以及了解宿主与病毒的相互作用。
