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一种用于检测半胱氨酸的高选择性喹嗪鎓基荧光探针。

A highly selective quinolizinium-based fluorescent probe for cysteine detection.

作者信息

O Wa-Yi, Chan Wing-Cheung, Xu Caifeng, Deng Jie-Ren, Ko Ben Chi-Bun, Wong Man-Kin

机构信息

The Hong Kong Polytechnic University, Shenzhen Research Institute Shenzhen P. R. China

State Key Laboratory of Chemical Biology and Drug Discovery, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University Hung Hom Hong Kong P. R. China

出版信息

RSC Adv. 2021 Oct 11;11(53):33294-33299. doi: 10.1039/d1ra06104f. eCollection 2021 Oct 8.

DOI:10.1039/d1ra06104f
PMID:35497514
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9042279/
Abstract

A novel fluorescent quinolizinium-based turn-off probe has been developed for selective detection of cysteine. The probe showed high selectivity and sensitivity towards cysteine over other amino acids including the similarly structured homocysteine and glutathione with a detection limit of 0.18 μM (S/N = 3). It was successfully applied to cysteine detection in living cells with low cytotoxicity and quantitative analysis of spiked mouse serum samples with moderate to good recovery (96-109%).

摘要

一种新型的基于喹嗪鎓的荧光猝灭探针已被开发用于选择性检测半胱氨酸。该探针对半胱氨酸显示出高于其他氨基酸（包括结构相似的同型半胱氨酸和谷胱甘肽）的高选择性和灵敏度，检测限为0.18 μM（信噪比 = 3）。它已成功应用于活细胞中的半胱氨酸检测，具有低细胞毒性，并用于加标小鼠血清样品的定量分析，回收率中等至良好（96 - 109%）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da24/9042279/6fd65224899a/d1ra06104f-f7.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da24/9042279/042b0b095f3e/d1ra06104f-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da24/9042279/e38a43276383/d1ra06104f-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da24/9042279/e89fc43fedfb/d1ra06104f-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da24/9042279/4844e09054eb/d1ra06104f-s2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da24/9042279/2c5685e970e1/d1ra06104f-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da24/9042279/6fd65224899a/d1ra06104f-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da24/9042279/3f8cc7b1ec63/d1ra06104f-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da24/9042279/ccd350ec320c/d1ra06104f-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da24/9042279/971d762cb225/d1ra06104f-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da24/9042279/042b0b095f3e/d1ra06104f-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da24/9042279/e38a43276383/d1ra06104f-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da24/9042279/e89fc43fedfb/d1ra06104f-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da24/9042279/4844e09054eb/d1ra06104f-s2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da24/9042279/2c5685e970e1/d1ra06104f-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da24/9042279/6fd65224899a/d1ra06104f-f7.jpg

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