采血技术、抗凝剂和储存温度对多形核中性粒细胞的分离影响较小。

Blood collection technique, anticoagulants and storing temperature have minor effects on the isolation of polymorphonuclear neutrophils.

机构信息

Institute of Occupational, Social and Environmental Medicine, Medical Faculty, RWTH Aachen University, Pauwelsstraße 30, 52074, Aachen, Germany.

Institute of Pharmacology and Toxicology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074, Aachen, Germany.

出版信息

Sci Rep. 2020 Sep 4;10(1):14646. doi: 10.1038/s41598-020-71500-1.

In the isolation of polymorphonuclear neutrophils (PMNs) the technique and other external factors can have great influence on the quality and quantity of isolated neutrophils. To elucidate the influence of the blood collection technique, anticoagulants and storing temperature on isolated PMNs healthy volunteers provided blood samples with different needles and collection techniques, anticoagulants (EDTA, heparin, citrate) and storing temperatures (4, 22, 37 °C). From each blood sample PMNs were isolated and compared regarding number of PMNs and oxidative burst. The blood collection technique, anticoagulants and storing temperature had minor impact on isolated PMNs. All three tested cannulas and anticoagulants can be used to obtain blood samples for PMN isolation. For storing temperatures 37 °C should be preferred. Regarding time between the PMN isolation and the actual experiments, a time span of maximum 1 h should be targeted.

在分离多形核中性粒细胞（PMN）时，技术和其他外部因素会对分离出的PMN 的质量和数量产生很大的影响。为了阐明采血技术、抗凝剂和储存温度对分离出的 PMN 的影响，健康志愿者使用不同的针头和采血技术、抗凝剂（EDTA、肝素、柠檬酸盐）以及储存温度（4°C、22°C、37°C）提供了血液样本。从每份血液样本中分离出 PMN，并比较 PMN 的数量和氧化爆发情况。采血技术、抗凝剂和储存温度对分离出的 PMN 影响较小。三种测试的套管和抗凝剂均可用于获得用于 PMN 分离的血液样本。储存温度应首选 37°C。至于 PMN 分离和实际实验之间的时间间隔，目标时间跨度应最大为 1 小时。