Liu Juan, Gao Jia-Qi, Chen Su-Yi-le, Jiang Chao, Yuan Yuan, Jiao Shun-Gang, Meng Hu-Biao, Chai Xing-Yun, Huang Lu-Qi
National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China School of Pharmacy, Jiangsu University Zhenjiang 212013, China.
Zhongguo Zhong Yao Za Zhi. 2020 Aug;45(15):3651-3658. doi: 10.19540/j.cnki.cjcmm.20200527.106.
As an important substitute for agarwood, mountain-agarwood, belonging to the family Oleaceae, comes from the root, stem and thick branch of Syringa pinnatifolia, which has a wide range of application in Inner Mongolia, China. It has good clinical efficacy in the use of cardiovascular diseases. However, the formation speed of mountain-agarwood is extremely slow, and its cultivated seedlings have low resin content. Therefore, how to speed up the formation of mountain-agarwood and increase the resin content is a hot research topic in this field. In this work, 16 S rDNA amplicon sequencing method was used to systematically analyze the bacterial communities of different samples of mountain-agarwood. Our data revealed that the samples of mountain-agarwood had more obvious species diversity than the ones of non-mountain-agarwood, especially the wild mountain-agarwood samples. By analysis of bacterial community composition and species abundance, Sphingomonas, Modestobacter and unidentified Cyanobacteria genus were three dominant bacterial genera in all samples. In addition, there are two identified genera of dominant bacteria, namely Actinoplanes and Microbacterium in both wild and cultivated mountain-agarwood, by bacterial community composition and species richness analysis. Meanwhile, Roseomonas was the dominant bacterial genus in both wild and cultivated non-mountain-agarwood samples. Our work could provides basic data for exploring the mechanism of the mountain-agarwood formation, and help to exploit resource of endophytic bacteria reasonably.
山沉香作为沉香的重要替代品，属于木犀科，来源于羽叶丁香的根、茎和粗枝，在中国内蒙古有着广泛的应用。它在心血管疾病的治疗中具有良好的临床疗效。然而，山沉香的形成速度极其缓慢，其人工培育的幼苗树脂含量较低。因此，如何加快山沉香的形成并提高其树脂含量是该领域研究的热点课题。在本研究中，采用16S rDNA扩增子测序方法对山沉香不同样品的细菌群落进行了系统分析。我们的数据显示，山沉香样品比非山沉香样品具有更明显的物种多样性，尤其是野生山沉香样品。通过对细菌群落组成和物种丰度的分析，鞘氨醇单胞菌属、适度杆菌属和未鉴定的蓝细菌属是所有样品中的三个优势细菌属。此外，通过细菌群落组成和物种丰富度分析，在野生和人工培育的山沉香中均有两个已鉴定的优势细菌属，即游动放线菌属和微杆菌属。同时，玫瑰单胞菌属是野生和人工培育的非山沉香样品中的优势细菌属。我们的研究可为探索山沉香形成机制提供基础数据，并有助于合理开发内生细菌资源。
