Zhang Xiang, Gao Jia-Qi, Yuan Yuan, Yang Quan, Zhou Liang-Yun, Liu Juan, Huang Lu-Qi
School of Traditional Chinese Medicine, Guangdong Pharmaceutical University Guangzhou 510006, China.
School of Pharmacy, Jiangsu University Zhenjiang 212013, China.
Zhongguo Zhong Yao Za Zhi. 2020 May;45(10):2374-2381. doi: 10.19540/j.cnki.cjcmm.20200302.115.
To explore the diversity of bacterial community structure between different layers of agarwood, Hiseq(high-throughput sequencing) was used to analyze the bacterial community structure of samples from different layers of agarwood. Our results showed that 1 150 096 optimized sequences and 9 690 OTUs were obtained from 15 samples of 5 layers of agarwood, which belonged to 28 bacterial phyla, 61 classes, 110 orders, 212 families and 384 genera. Further analysis revealed that the normal layer(NL) had the lowest bacterial species richness and the smallest number of OTUs. And the total number of OTUs of the agarwood layer(AL) and NL was zero, which was quite different.At the same time, there were significant differences in bacterial community structure and species diversity between NL and the other four layers. While there were some common dominant bacterial genera in both transition layer(TL) and NL. The similarity of bacterial distribution in 4 non-NL layers was relatively high, which had four common genera, such as Acidibacter, Bradyrhizobium, Acidothemus and Sphingomonas. While Acidibacter, Bradyrhizobium and Acidothemus were the dominant bacterial genus of DA and AL, and all of these layers contained volatile oil. In addition, the Bradyrhizobium was the most abundant in agarwood layer. Our results showed that bacterial community diversity and abundance were decreasing from DL to AL, and different layers showed significant differences in bacterial enrichment. It provided the clues to investigate how bacteria participate in the formation of agarwood.
为探究沉香不同层次间细菌群落结构的多样性，采用Hiseq（高通量测序）分析沉香不同层次样品的细菌群落结构。结果显示，从5层沉香的15个样品中获得了1150096条优化序列和9690个OTU，它们隶属于28个细菌门、61个纲、110个目、212个科和384个属。进一步分析发现，正常层（NL）的细菌物种丰富度最低，OTU数量最少。且沉香层（AL）和NL的OTU总数为零，差异显著。同时，NL与其他四层之间的细菌群落结构和物种多样性存在显著差异。而过渡层（TL）和NL中存在一些共同的优势细菌属。4个非NL层的细菌分布相似性较高，有4个共同的属，如嗜酸菌属、慢生根瘤菌属、嗜酸嗜热放线菌属和鞘氨醇单胞菌属。嗜酸菌属、慢生根瘤菌属和嗜酸嗜热放线菌属是腐朽层（DA）和AL的优势细菌属，且这些层均含有挥发油。此外，慢生根瘤菌属在沉香层中最为丰富。结果表明，细菌群落多样性和丰度从腐朽层（DL）到AL逐渐降低，不同层次在细菌富集方面表现出显著差异。这为研究细菌如何参与沉香的形成提供了线索。
