Wu Gao-Song, Zhong Jing, Zheng Ning-Ning, Wang Chao-Ran, Jin Hong-Li, Ge Guang-Bo, Han Jing-Yan, Gao Yue, Sheng Li-Li, Zhang Wei-Dong, Li Hou-Kai
Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine Shanghai 201203, China.
Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine Shanghai 201203, China Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital, Affiliated Central Hospital of Huzhou University Huzhou 313000, China.
Zhongguo Zhong Yao Za Zhi. 2020 Aug;45(15):3726-3739. doi: 10.19540/j.cnki.cjcmm.20200609.201.
This study is to explore the effect of Qingfei Paidu Decoction(QPD) on the host metabolism and gut microbiome of rats with metabolomics and 16 S rDNA sequencing. Based on 16 S rDNA sequencing of gut microbiome and metabolomics(GC-MS and LC-MS/MS), we systematically studied the serum metabolites profile and gut microbiota composition of rats treated with QPD for continued 5 days by oral gavage. A total of 23 and 43 differential metabolites were identified based on QPD with GC-MS and LC-MS/MS, respectively. The involved metabolic pathways of these differential metabolites included glycerophospholipid metabolism, linoleic acid metabolism, TCA cycle and pyruvate metabolism. Meanwhile, we found that QPD significantly regulated the composition of gut microbiota in rats, such as enriched Romboutsia, Turicibacter, and Clostridium_sensu_stricto_1, and decreased norank_f_Lachnospiraceae. Our current study indicated that short-term intervention of QPD could significantly regulate the host metabolism and gut microbiota composition of rats dose-dependently, suggesting that the clinical efficacy of QPD may be related with the regulation on host metabolism and gut microbiome.
本研究旨在通过代谢组学和16S rDNA测序探讨清肺排毒汤（QPD）对大鼠宿主代谢和肠道微生物群的影响。基于肠道微生物群的16S rDNA测序和代谢组学（气相色谱-质谱联用仪和液相色谱-串联质谱），我们系统地研究了通过灌胃连续5天给予QPD治疗的大鼠的血清代谢物谱和肠道微生物群组成。基于气相色谱-质谱联用仪和液相色谱-串联质谱分别鉴定出23种和43种差异代谢物。这些差异代谢物涉及的代谢途径包括甘油磷脂代谢、亚油酸代谢、三羧酸循环和丙酮酸代谢。同时，我们发现QPD显著调节大鼠肠道微生物群的组成，如使罗姆布茨菌属、Turicibacter菌属和严格梭菌1群富集，并减少了未分类的毛螺菌科。我们目前的研究表明，QPD的短期干预可显著剂量依赖性地调节大鼠的宿主代谢和肠道微生物群组成，提示QPD的临床疗效可能与对宿主代谢和肠道微生物群的调节有关。
