Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior 474 002, India.
J Genet. 2020;99.
This study was undertaken to measure the genetic diversity and population structure of 48 barley accessions introduced from ICARDA using 51 polymorphic simple sequence repeat (SSR) markers to select unique parents for breeding. The mean polymorphic information content was 0.491, suggesting high polymorphism for the selected SSR markers among the barley accessions. The population structure indicated a fine genetic base only with two major clusters. All accessions had 100% membership probability in their respective clusters. Analysis of molecular variance revealed that most (78%) of the variation was attributed between populations, while 22% was due to variation among individuals within populations. Neighbour-joining (NJ) tree was constructed using this distance matrix and two major clusters were observed in it. Cluster 1 had all hulled barley accessions and cluster 2 had all hulless barley accessions. Cluster 2 could be further divided into three subclusters. Principal coordinates analysis results were similar to the NJ tree, where the hulled and hulless barley accessions were grouped into separate clusters. This study established the existence of considerable genetic diversity among the 48 tested accessions. The selected genetic resources will be useful for barley breeding in India and other countries.
本研究使用 51 个多态性简单序列重复 (SSR) 标记，对来自 ICARDA 的 48 个大麦引进品种进行遗传多样性和群体结构分析，旨在选择用于育种的独特亲本。平均多态信息含量为 0.491，表明所选 SSR 标记在大麦品种中具有高度多态性。群体结构仅显示出一个精细的遗传基础，仅有两个主要聚类。所有品种在各自的聚类中都有 100%的归属概率。分子方差分析表明，大部分（78%）变异归因于群体间，而 22%归因于群体内个体间的变异。使用这个距离矩阵构建了基于邻接法（NJ）的聚类树，观察到两个主要聚类。聚类 1 包含所有皮大麦品种，聚类 2 包含所有裸大麦品种。聚类 2 可以进一步细分为三个亚聚类。主坐标分析结果与 NJ 树相似，皮大麦和裸大麦品种被分组到不同的聚类中。本研究证实了 48 个受测品种之间存在相当大的遗传多样性。所选遗传资源将对印度和其他国家的大麦育种有用。
