Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
J Zhejiang Univ Sci B. 2010 Oct;11(10):792-800. doi: 10.1631/jzus.B0900414.
The genetic diversity and relationship among 40 elite barley varieties were analyzed based on simple sequence repeat (SSR) genotyping data. The amplified fragments from SSR primers were highly polymorphic in the barley accessions investigated. A total of 85 alleles were detected at 35 SSR loci, and allelic variations existed at 29 SSR loci. The allele number per locus ranged from 1 to 5 with an average of 2.4 alleles per locus detected from the 40 barley accessions. A cluster analysis based on the genetic similarity coefficients was conducted and the 40 varieties were classified into two groups. Seven malting barley varieties from China fell into the same subgroup. It was found that the genetic diversity within the Chinese malting barley varieties was narrower than that in other barley germplasm sources, suggesting the importance and feasibility of introducing elite genotypes from different origins for malting barley breeding in China.
基于简单序列重复（SSR）基因型数据，分析了 40 个精选大麦品种的遗传多样性和关系。在所研究的大麦品种中，SSR 引物扩增的片段高度多态性。在 35 个 SSR 位点共检测到 85 个等位基因，29 个 SSR 位点存在等位基因变异。每个位点的等位基因数范围为 1-5，从 40 个大麦品种中检测到每个位点的平均等位基因数为 2.4 个。基于遗传相似系数的聚类分析将 40 个品种分为两组。来自中国的 7 个麦芽大麦品种归入同一亚组。研究发现，中国麦芽大麦品种的遗传多样性比其他大麦种质资源窄，这表明从不同来源引入优良基因型对中国麦芽大麦育种具有重要性和可行性。
