利用与初级和次级代谢物相关的 SSR 和 ISSR 标记对大麦（Hordeum vulgare L.）在热胁迫条件下进行关联分析、遗传多样性和群体结构分析。

Association analysis, genetic diversity and population structure of barley (Hordeum vulgare L.) under heat stress conditions using SSR and ISSR markers linked to primary and secondary metabolites.

机构信息

Department of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Persian Gulf Highway, P.O. Box: 41635-1314, Rasht, Guilan, Iran.

Department of Plant Production, Faculty of Agriculture and Natural Resources, Gonbad University, Shahid Fallahi Street, Gonbad-e Kāvūs, Golestan, Iran.

出版信息

Mol Biol Rep. 2021 Oct;48(10):6673-6694. doi: 10.1007/s11033-021-06652-y. Epub 2021 Sep 8.

DOI:10.1007/s11033-021-06652-y

PMID:34495461

Abstract

BACKGROUND

Barley is one of the major cereal crops, which can provide a significant source of genes for stress tolerance due to its high diversity and adaptability. Metabolite traits are considered to be significant for adaptation of barley to heat stress.

METHODS AND RESULTS

In the present study, genetic relationships between 120 barley genotypes were determined with 50 simple sequence repeat (SSR) and 26 inter simple sequence repeat (ISSR) markers under heat stress and non-stress conditions. Moreover, genetic diversity of barley accessions was investigated using the studied markers covering 7 chromosomes of barley.

RESULTS

In general, 153 and 85 polymorphic alleles were detected for SSR and ISSR and number of the observed polymorphic allele varied between 2-9 and 2-6, with an average of 3.26 and 3.26 alleles per locus, respectively. Markers of Bmag0223, GBMS180/180, HVM7, ISSR22, ISSR25, and ISSR48 were the most informative due to their high polymorphism information content value demonstrating that putative techniques utilized in this research can be powerful and valuable tools in breeding program of barley. Association analysis was performed between 9 important traits and SSR and ISSR markers using four statistical models. The results revealed that the model containing both population structure (Q) and general similarity in genetic background arising from shared kinship (K) factors reduced false positive associations between markers and phenotypes.

CONCLUSIONS

According to the results, some of markers related to more than one trait under normal conditions (ISSR31-2, HVM62, and GBMS180/180) and heat stress conditions (ISSR20-5, EBmac635, HVM14, and ISSR37-3) were determined, which can be considered to be the most interesting candidates for further studies and simultaneously will provide a useful target for the future breeding programs, such as marker-assisted selection (MAS).

摘要

背景

大麦是主要的谷类作物之一，由于其多样性和适应性，可为其耐受胁迫提供丰富的基因资源。代谢性状被认为是大麦适应热胁迫的重要特征。

方法和结果

本研究在热胁迫和非胁迫条件下，使用 50 个简单序列重复（SSR）和 26 个简单重复间序列扩增多态性（ISSR）标记，对 120 个大麦基因型的遗传关系进行了测定。此外，利用所研究的标记，对包括大麦 7 条染色体在内的大麦种质资源的遗传多样性进行了研究。

结果

SSR 和 ISSR 共检测到 153 和 85 个多态性等位基因，观察到的多态性等位基因数量分别在 2-9 和 2-6 之间，平均每个位点分别有 3.26 和 3.26 个等位基因。Bmag0223、GBMS180/180、HVM7、ISSR22、ISSR25 和 ISSR48 等标记的多态性信息含量值较高，表明所采用的技术在大麦的遗传改良中具有很高的应用价值。利用 4 种统计模型，对 9 个重要性状与 SSR 和 ISSR 标记之间的关系进行了关联分析。结果表明，模型中包含群体结构（Q）和由共享亲缘关系（K）引起的遗传背景相似性因素，减少了标记与表型之间的假阳性关联。

结论

根据结果，确定了一些在正常条件下（ISSR31-2、HVM62 和 GBMS180/180）和热胁迫条件下（ISSR20-5、EBmac635、HVM14 和 ISSR37-3）与多个性状相关的标记，这些标记可以作为进一步研究的候选标记，同时也为未来的遗传改良计划（如标记辅助选择（MAS））提供了有用的目标。