School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, China.
Department of Pathology, The Second Xiangya Hospital, Central South University, Changsha, China.
FEBS Open Bio. 2020 Oct;10(10):2206-2215. doi: 10.1002/2211-5463.12971. Epub 2020 Sep 21.
Chromobox (CBX) family members are vital epigenetic regulators that repress the transcription of target genes through chromatin modification. Several studies have investigated the role of CBX family members in cancer. However, the function and prognostic value of diverse CBX family members in non-small-cell lung cancer remain largely unknown. In this study, we reveal that CBX family members are overexpressed in non-small-cell lung cancer tissue compared with normal lung tissue, with the exception of CBX6. Kaplan-Meier analysis demonstrated that high expressions of CBX1 and CBX3 are correlated with overall survival, disease-specific survival, disease-free interval, and progression-free interval for patients with lung adenocarcinoma (LUAD). Furthermore, regression model analysis suggests that CBX3 may be suitable as an independent prediction factor for overall survival and progression-free interval in patients with LUAD. In addition, CBX3 mRNA expression was found to be associated with tumor diameter and lymph node metastasis. Gene enrichment analysis suggests that CBX3 is involved in the cell cycle and P53 signaling pathways. Aberrant expression of CBX3 in LUAD is correlated with DNA copy number alteration. In summary, our data imply that CBX3 plays an important role in the promotion of LUAD and may thus have potential as a prognostic biomarker and molecular therapeutic target for the disease.
染色盒（CBX）家族成员是重要的表观遗传调控因子，通过染色质修饰抑制靶基因的转录。已有多项研究探讨了 CBX 家族成员在癌症中的作用。然而，不同 CBX 家族成员在非小细胞肺癌中的功能和预后价值仍知之甚少。在本研究中，我们发现与正常肺组织相比，非小细胞肺癌组织中 CBX 家族成员表达上调，除 CBX6 外。Kaplan-Meier 分析表明，CBX1 和 CBX3 的高表达与肺腺癌（LUAD）患者的总生存期、疾病特异性生存期、无病间期和无进展间期相关。此外，回归模型分析表明，CBX3 可能是 LUAD 患者总生存期和无进展间期的独立预测因子。此外，CBX3mRNA 表达与肿瘤直径和淋巴结转移相关。基因富集分析表明，CBX3 参与细胞周期和 P53 信号通路。LUAD 中 CBX3 的异常表达与 DNA 拷贝数改变有关。总之，我们的数据表明 CBX3 在 LUAD 的促进中起重要作用，因此可能具有作为疾病的预后生物标志物和分子治疗靶点的潜力。
