Institute for Bioinformatics and Evolutionary Studies (IBEST), University of Idaho, Moscow, ID, 83844, USA.
Computational Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA.
Commun Biol. 2020 Sep 7;3(1):489. doi: 10.1038/s42003-020-01169-9.
The larvae of click beetles (Coleoptera: Elateridae), known as "wireworms," are agricultural pests that pose a substantial economic threat worldwide. We produced one of the first wireworm genome assemblies (Limonius californicus), and investigated population structure and phylogenetic relationships of three species (L. californicus, L. infuscatus, L. canus) across the northwest US and southwest Canada using genome-wide markers (RADseq) and genome skimming. We found two species (L. californicus and L. infuscatus) are comprised of multiple genetically distinct groups that diverged in the Pleistocene but have no known distinguishing morphological characters, and therefore could be considered cryptic species complexes. We also found within-species population structure across relatively short geographic distances. Genome scans for selection provided preliminary evidence for signatures of adaptation associated with different pesticide treatments in an agricultural field trial for L. canus. We demonstrate that genomic tools can be a strong asset in developing effective wireworm control strategies.
叩头虫（鞘翅目：叩甲科）的幼虫，俗称“铁丝虫”，是一种对农业具有重大经济威胁的害虫。我们首次组装了一种铁丝虫基因组（加利福尼亚叩头虫），并利用全基因组标记（RADseq）和基因组刮削技术，研究了美国西北部和加拿大西南部的 3 个物种（加利福尼亚叩头虫、暗叩头虫和黑叩头虫）的种群结构和系统发育关系。我们发现有两个物种（加利福尼亚叩头虫和暗叩头虫）由多个在更新世分化但没有明显形态特征的遗传上明显不同的群体组成，因此可以被认为是隐种复合体。我们还发现，在相对较短的地理距离内存在种内种群结构。对选择的基因组扫描为与加利福尼亚叩头虫田间农业试验中不同农药处理相关的适应特征提供了初步证据。我们证明，基因组工具在制定有效的铁丝虫防治策略方面具有强大的优势。
