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由市售部件组装而成的低成本自动毛细管电泳仪。

Low-cost automated capillary electrophoresis instrument assembled from commercially available parts.

作者信息

Furter Jasmine S, Boillat Marc-Aurèle, Hauser Peter C

机构信息

Department of Chemistry, University of Basel, Basel, Switzerland.

出版信息

Electrophoresis. 2020 Dec;41(24):2075-2082. doi: 10.1002/elps.202000211. Epub 2020 Sep 21.

Abstract

A CE instrument that can be assembled from commercially available components with minimal construction effort is described. Except for the electronic control circuitry no specially made parts are required. It is based on a flexible design of microfluidic, electropneumatic, and electronic sections and different configurations can easily be implemented. Automated injection into the capillary is performed hydrodynamically by the application of a pressure for a controlled length of time. The performance of the device was tested with a contactless conductivity detector by separating different metal ions. In addition, nine metal cations related to the quality of honey were separated in 2.3 min and four honey samples were analysed quantitatively to demonstrate the applicability of the method.

摘要

本文描述了一种可以用市售部件以最少的组装工作量组装而成的毛细管电泳仪。除电子控制电路外,无需特别制作的部件。它基于微流体、电动气动和电子部分的灵活设计,不同配置可轻松实现。通过在受控时间内施加压力,以流体动力学方式自动将样品注入毛细管。该装置的性能通过使用非接触式电导检测器分离不同金属离子进行测试。此外,在2.3分钟内分离了与蜂蜜质量相关的九种金属阳离子,并对四个蜂蜜样品进行了定量分析,以证明该方法的适用性。

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