Kitano Y
Department of Dermatology, Osaka University School of Medicine, Japan.
Cell Biol Int Rep. 1988 Mar;12(3):189-94. doi: 10.1016/0309-1651(88)90095-1.
Distribution of actin filaments in human malignant keratinocytes was examined by immunofluorescence staining. The primary cultures were obtained from a squamous cell carcinoma, a basal cell carcinoma, and Bowen's disease. Rhodamine-phalloidin staining revealed that actin filaments were occasionally organized to form stress fibers, many short bundles with a ripple appearance, and regular arrays of actin patches. Some of these structures appeared in untransformed keratinocytes as a result of a brief exposure to a tumor promotor, TPA. These findings suggest that regulation of actin functions is involved in neoplastic processes from the very early stages and that alteration is persistent in neoplastic cells.
通过免疫荧光染色检查了人恶性角质形成细胞中肌动蛋白丝的分布。原代培养物取自鳞状细胞癌、基底细胞癌和鲍温病。罗丹明-鬼笔环肽染色显示，肌动蛋白丝偶尔会组织形成应力纤维、许多呈波纹状外观的短束以及规则排列的肌动蛋白斑。这些结构中的一些会因短暂暴露于肿瘤促进剂佛波酯（TPA）而出现在未转化的角质形成细胞中。这些发现表明，肌动蛋白功能的调节从早期阶段就参与了肿瘤形成过程，并且这种改变在肿瘤细胞中持续存在。
