Graem N
Acta Pathol Microbiol Immunol Scand A. 1982 Nov;90(6):455-62. doi: 10.1111/j.1699-0463.1982.tb00122_90a.x.
Membrane changes in keratinocytes were studied in a selected series of skin biopsies from 58 patients comprising cases of healing wounds, keratoachanthomas, actinic keratoses, Bowen's disease and squamous and basal cell carcinomas. The changes were demonstrated by means of a fluorescein-conjugated lectin ricinus communis agglutinin I, which specifically binds to beta-D-galactopyranosyl residues normally present on the keratinocyte surface. The RCA I binding equalled the binding of the normal epidermis in hyperplastic epidermis adjacent to healing wounds and in keratoachanthomas, but was slightly decreased in actinic keratoses and cases of Bowen's disease. In epidermal outgrowths from the edges of healing wounds and in squamous and basal cell carcinomas a heavy loss of RCA I binding was seen. The results are supported by previous in vivo and in vitro studies of normal and transformed cells, and it is suggested that the presented histochemical RCA I binding technique could be a valuable diagnostic tool.
在对58例患者进行的一系列选定皮肤活检中研究了角质形成细胞的膜变化，这些患者包括愈合伤口、角化棘皮瘤、光化性角化病、鲍温病以及鳞状和基底细胞癌病例。通过荧光素偶联的凝集素蓖麻凝集素I证实了这些变化，该凝集素特异性结合通常存在于角质形成细胞表面的β-D-吡喃半乳糖基残基。在愈合伤口附近的增生性表皮和角化棘皮瘤中，RCA I结合与正常表皮的结合相当，但在光化性角化病和鲍温病病例中略有下降。在愈合伤口边缘的表皮增生以及鳞状和基底细胞癌中，可见RCA I结合大量丧失。这些结果得到了先前对正常细胞和转化细胞的体内和体外研究的支持，并且表明所提出的组织化学RCA I结合技术可能是一种有价值的诊断工具。
