Zhou Xiyi, Pu Hongbin, Sun Da-Wen
School of Food Science and Engineering, South China University of Technology, Guangzhou, China.
Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou, China.
Crit Rev Food Sci Nutr. 2021;61(14):2277-2296. doi: 10.1080/10408398.2020.1809343. Epub 2020 Sep 8.
The frequent occurrence of food safety incidents has given rise to unprecedented concern about food contamination issues for both consumers and the industry. Various contaminations in food pose serious threats to food safety and human health. Many detection methods were studied to address the challenge. Recently, biosensors relying on deoxyribonucleic acid (DNA)-functionalized nanoparticles have been developed as an efficient and effective detection method. In the current review, the strategies for DNA assembly metal and metal oxide nanoparticles are elaborated, recent applications of the sensors based on DNA-functionalized nanoparticles in food contaminant detection are discussed. Pathogenic bacteria, heavy metal ions, mycotoxins, antibiotics, and pesticides are covered as food contaminants. Additionally, limitations and future trends of functionalized nanoparticles-based technology are also presented. The current review indicates that DNA-functionalized metal and metal oxide nanoparticles are a novel nanomaterial with unique biological and physical properties for developing electrochemical, fluorescent, colourimetric and surface-enhanced Raman spectroscopy (SERS) sensors, etc. Compared with conventional detection techniques, DNA-functionalized metal and metal oxide nanoparticles have considerable advantages with high accuracy, high specificity, micro-intelligence, and low cost. Nevertheless, the stability of these sensors and the limitations of real-time detection are still under discussion. Therefore, more tolerant, portable, and rapid DNA sensors should be developed to better the real-time monitoring of harmful contaminants.
食品安全事件的频繁发生引发了消费者和食品行业对食品污染问题前所未有的关注。食品中的各种污染对食品安全和人类健康构成严重威胁。人们研究了许多检测方法来应对这一挑战。最近，基于脱氧核糖核酸（DNA）功能化纳米颗粒的生物传感器已被开发成为一种高效的检测方法。在当前的综述中，阐述了DNA组装金属和金属氧化物纳米颗粒的策略，讨论了基于DNA功能化纳米颗粒的传感器在食品污染物检测中的最新应用。作为食品污染物，涵盖了致病细菌、重金属离子、霉菌毒素、抗生素和农药。此外，还介绍了基于功能化纳米颗粒技术的局限性和未来趋势。当前的综述表明，DNA功能化金属和金属氧化物纳米颗粒是一种新型纳米材料，具有独特的生物学和物理特性，可用于开发电化学、荧光、比色和表面增强拉曼光谱（SERS）传感器等。与传统检测技术相比，DNA功能化金属和金属氧化物纳米颗粒具有高精度、高特异性、微智能和低成本等显著优势。然而，这些传感器的稳定性和实时检测的局限性仍在讨论中。因此，应开发更具耐受性、便携性和快速性的DNA传感器，以更好地实时监测有害污染物。
