Genome-wide identification of genes in and their expression under different nitrogen treatments.

UNLABELLED

Fructokinase (FRK) is the main fructose phosphorylase and plays an important role in catalyzing the irreversible reaction of free fructose phosphorylation. In order to study the regulatory effect of different forms and concentrations of nitrogen on genes in , seven genes encoding the hypothetical FRK proteins were identified in genome by bioinformatics method. Phylogenetic analysis revealed that family genes can be divided into two subgroups: SI () and SII (). The tissue-specific expression data obtained from PopGenIE indicate that and are expressed highly in the stem. Quantitative real-time RT-PCR illustrate that showed different expression patterns in different tissues under different concentrations and morphological nitrogen application. Under high nitrate treatment, the expression levels of and in stem increased significantly, while under low nitrate treatment, only the expression of in the upper stem and the expression of in the lower stem increased significantly. In contrast, ammonium tends to inhibit the expression of in lower stems, the expression levels of and are significantly reduced under ammonium treatment. However, high ammonium had significant effects on in the apical bud and upper leaves, which were 6 and 8 times of the control, respectively. These results laid the foundation for the study of the gene family of poplar and provided a theoretical basis for the molecular mechanism of nitrogen regulating cell wall development.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s12298-021-01055-6.