Zuo Zhuang, Sun Xue, Cao Lina, Zhang Shuang, Yu Jiajie, Xu Xiuyue, Xu Zhiru, Liu Guanjun, Qu Chunpu
State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry University, Harbin, 150040 People's Republic of China.
School of Forestry, Northeast Forestry University, Harbin, 150040 People's Republic of China.
Physiol Mol Biol Plants. 2021 Sep;27(9):1919-1931. doi: 10.1007/s12298-021-01055-6. Epub 2021 Sep 13.
Fructokinase (FRK) is the main fructose phosphorylase and plays an important role in catalyzing the irreversible reaction of free fructose phosphorylation. In order to study the regulatory effect of different forms and concentrations of nitrogen on genes in , seven genes encoding the hypothetical FRK proteins were identified in genome by bioinformatics method. Phylogenetic analysis revealed that family genes can be divided into two subgroups: SI () and SII (). The tissue-specific expression data obtained from PopGenIE indicate that and are expressed highly in the stem. Quantitative real-time RT-PCR illustrate that showed different expression patterns in different tissues under different concentrations and morphological nitrogen application. Under high nitrate treatment, the expression levels of and in stem increased significantly, while under low nitrate treatment, only the expression of in the upper stem and the expression of in the lower stem increased significantly. In contrast, ammonium tends to inhibit the expression of in lower stems, the expression levels of and are significantly reduced under ammonium treatment. However, high ammonium had significant effects on in the apical bud and upper leaves, which were 6 and 8 times of the control, respectively. These results laid the foundation for the study of the gene family of poplar and provided a theoretical basis for the molecular mechanism of nitrogen regulating cell wall development.
The online version contains supplementary material available at 10.1007/s12298-021-01055-6.
果糖激酶（FRK）是主要的果糖磷酸化酶，在催化游离果糖磷酸化的不可逆反应中起重要作用。为了研究不同形态和浓度的氮对[物种名称]中基因的调控作用，通过生物信息学方法在[物种名称]基因组中鉴定出7个编码假定FRK蛋白的基因。系统发育分析表明，[物种名称]家族基因可分为两个亚组：SI（[亚组I名称]）和SII（[亚组II名称]）。从PopGenIE获得的组织特异性表达数据表明，[基因名称1]和[基因名称2]在茎中高表达。定量实时RT-PCR表明，在不同浓度和形态的氮处理下，[基因名称]在不同组织中表现出不同的表达模式。在高硝酸盐处理下，茎中[基因名称1]和[基因名称2]的表达水平显著增加，而在低硝酸盐处理下，仅上茎中[基因名称3]的表达和下茎中[基因名称4]的表达显著增加。相反，铵倾向于抑制下茎中[基因名称5]的表达，在铵处理下[基因名称6]和[基因名称7]的表达水平显著降低。然而，高铵对顶芽和上部叶片中的[基因名称8]有显著影响，分别是对照的6倍和8倍。这些结果为杨树[基因名称]家族的研究奠定了基础，并为氮调节细胞壁发育的分子机制提供了理论依据。
在线版本包含可在10.1007/s12298-021-01055-6获取的补充材料。
