Luo Dandan, Wang Peng, Lu Huifei, Zeng Xianhai, Zhao Hailiang, Liu Jiangqi
Department of Operating Room, The Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China.
Department of Graduate and Scientific Research, Zhuhai Campus of Zunyi Medical University, Zhuhai, China.
Medicine (Baltimore). 2025 Jun 6;104(23):e42645. doi: 10.1097/MD.0000000000042645.
The escalating prevalence of allergic diseases has intensified the need for rapid and precise allergen detection, prompting the exploration of microfluidic technology in this domain. This review delves into the utility of microfluidics in enhancing allergen detection, underscoring its high sensitivity, throughput, and miniaturization advantages. We survey the spectrum of microfluidic chip materials, from silicon wafers to paper-based substrates, and discuss various fabrication techniques. The integration of microfluidics with detection methods like enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction, Western blotting, loop-mediated isothermal amplification (LAMP), and biosensors has transformed allergen diagnostics, offering more efficient, sensitive, and user-friendly platforms. These advancements facilitate point-of-care testing and hold significant potential for personalized medicine. Our review highlights the novel applications of microfluidics in allergen detection, contributing valuable insights for the development of future diagnostic tools and clinical research in allergy treatment. The innovative convergence of microfluidics with existing diagnostic methods presents a paradigm shift in the field of allergen detection, promising enhanced prevention and management of allergic diseases.
过敏性疾病患病率的不断上升，加剧了对快速、精确过敏原检测的需求，促使人们在该领域探索微流控技术。本综述深入探讨了微流控技术在增强过敏原检测方面的效用，强调了其高灵敏度、高通量和小型化优势。我们考察了从硅片到纸质基底等微流控芯片材料的范围，并讨论了各种制造技术。微流控技术与酶联免疫吸附测定（ELISA）、聚合酶链反应、蛋白质印迹法、环介导等温扩增（LAMP）和生物传感器等检测方法的整合，已经改变了过敏原诊断，提供了更高效、灵敏且用户友好的平台。这些进展促进了即时检测，并在个性化医疗方面具有巨大潜力。我们的综述突出了微流控技术在过敏原检测中的新应用，为未来诊断工具的开发以及过敏治疗的临床研究提供了有价值的见解。微流控技术与现有诊断方法的创新性融合，在过敏原检测领域带来了范式转变，有望加强对过敏性疾病的预防和管理。
