Salfinger M, Stool E W, Piot D, Heifets L
National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.
J Clin Microbiol. 1988 Jun;26(6):1225-6. doi: 10.1128/jcm.26.6.1225-1226.1988.
Three methods were used for the recovery of mycobacteria from blood specimens obtained from acquired immunodeficiency syndrome patients: (i) inoculation of 7H11 agar plates with a concentrated specimen, (ii) inoculation of 7H12 BACTEC vials with a concentrated specimen, and (iii) inoculation of 7H13 BACTEC vials with a nonconcentrated specimen. In this study, we examined 255 specimens and obtained positive mycobacterial growth in 47 of them. Among these 47 cultures, 40 were found to be positive by all three methods, and the total recovery rates in relation to these culture-positive specimens were 94% for method 1, 89% for method 2, and 96% for method 3. The advantages and disadvantages of these three methods are discussed.
(i)用浓缩标本接种7H11琼脂平板,(ii)用浓缩标本接种7H12 BACTEC瓶,(iii)用未浓缩标本接种7H13 BACTEC瓶。在本研究中,我们检测了255份标本,其中47份获得了分枝杆菌阳性生长。在这47份培养物中,40份通过所有三种方法均呈阳性,对于这些培养阳性标本,方法1的总回收率为94%,方法2为89%,方法3为96%。讨论了这三种方法的优缺点。