Damato J J, Collins M T, Rothlauf M V, McClatchy J K
J Clin Microbiol. 1983 Jun;17(6):1066-73. doi: 10.1128/jcm.17.6.1066-1073.1983.
A group of 89 smear-positive sputum specimens were evaluated by radiometric and standard plate procedures to determine the methodology which would provide the earliest detection of mycobacteria and maximum test sensitivity. Digested non-decontaminated specimens were concentrated and inoculated into modified selective BACTEC radiometric 7H12 broth and Mitchison selective 7H10 agar. Sodium hydroxide (1.5% final concentration) was then used to decontaminate these specimens. They were then concentrated and inoculated into both selective and nonselective 7H12 radiometric broths and into selective 7H10 and nonselective Middlebrook 7H11 agar media. The specimen processing and media combinations providing the earliest detection were non-decontaminated specimens with modified selective 7H12 BACTEC broth and decontaminated specimens with 7H12 BACTEC broths. Maximum sensitivity (percent positive) was obtained by using non-decontaminated specimens on Mitchison selective 7H10 Agar (98%) or decontaminated specimens in 7H12 BACTEC broth (95%). The decontamination process was found to reduce significantly the number of mycobacteria in clinical specimens, particularly the mycobacteria other than Mycobacterium tuberculosis. The specimen processing-media combinations providing the earliest detection and maximum recovery of mycobacteria (100%) were non-decontaminated specimens with modified selective 7H12 BACTEC broth or Mitchison selective agar and decontaminated specimens with 7H12 BACTEC broth or 7H11 agar.
对89份涂片阳性痰标本采用放射性测量法和标准平板法进行评估，以确定能最早检测出分枝杆菌并具有最大检测灵敏度的方法。将消化后的未净化标本浓缩后接种于改良选择性BACTEC放射性7H12肉汤和米奇森选择性7H10琼脂中。然后用氢氧化钠（终浓度1.5%）对这些标本进行净化处理。接着将它们浓缩并接种于选择性和非选择性7H12放射性肉汤以及选择性7H10和非选择性Middlebrook 7H11琼脂培养基中。能最早检测出分枝杆菌的标本处理和培养基组合是用改良选择性7H12 BACTEC肉汤处理的未净化标本以及用7H12 BACTEC肉汤处理的净化标本。通过在米奇森选择性7H10琼脂上使用未净化标本（98%）或在7H12 BACTEC肉汤中使用净化标本（95%）可获得最大灵敏度（阳性百分比）。发现净化过程能显著减少临床标本中分枝杆菌的数量，尤其是结核分枝杆菌以外的分枝杆菌。能最早检测出分枝杆菌并实现最大回收率（100%）的标本处理 - 培养基组合是用改良选择性7H12 BACTEC肉汤或米奇森选择性琼脂处理的未净化标本以及用7H12 BACTEC肉汤或7H11琼脂处理的净化标本。
