Division of Cardiology, Department of Internal Medicine, Chang Gung Memorial Hospital-Linkou, Chang Gung University College of Medicine, Tao-Yuan 33305, Taiwan.
Department of Thoracic and Cardiovascular Surgery, Chang Gung Memorial Hospital, Tao-Yuan 33305, Taiwan.
ACS Infect Dis. 2020 Oct 9;6(10):2688-2697. doi: 10.1021/acsinfecdis.0c00321. Epub 2020 Sep 28.
More than half of diabetic wounds demonstrate clinical signs of infection at presentation and lead to poor outcomes. This work develops coaxial sheath-core nanofibrous poly(lactide--glycolide) (PLGA) scaffolds that are loaded with bioactive antibiotics and platelet-derived growth factor (PDGF) for the repair of diabetic infectious wounds. PDGF and PLGA/antibiotic solutions were pumped, respectively, into two independent capillary tubings for coaxial electrospinning to prepare biodegradable sheath-core nanofibers. Spun nanofibrous scaffolds sustainably released PDGF, vancomycin, and gentamicin for 3 weeks. The scaffolds also reduced the phosphatase and tensin homologue content, enhanced the amount of angiogenesis marker (CD31) around the wound area, and accelerated healing in the early stage of infected diabetic wound repair. Antibiotic/biomolecule-loaded PLGA nanofibers may provide a very effective way to aid tissue regeneration at the sites of infected diabetic wounds.
超过一半的糖尿病伤口在就诊时表现出感染的临床迹象，导致不良后果。这项工作开发了同轴鞘芯纳米纤维聚（乳酸-乙醇酸）（PLGA）支架，该支架负载有生物活性抗生素和血小板衍生生长因子（PDGF），用于修复糖尿病感染性伤口。PDGF 和 PLGA/抗生素溶液分别被泵入两个独立的毛细管中进行同轴静电纺丝，以制备可生物降解的鞘芯纳米纤维。纺制的纳米纤维支架可持续释放 PDGF、万古霉素和庆大霉素长达 3 周。支架还降低了磷酸酶和张力蛋白同源物的含量，增加了伤口区域周围的血管生成标记物（CD31）的数量，并加速了感染性糖尿病伤口修复的早期愈合。载抗生素/生物分子的 PLGA 纳米纤维可能为感染性糖尿病伤口部位的组织再生提供一种非常有效的方法。
