Chen Chen, Han Hui, Xu Ting, Lv Yizhong, Hu Kelin, Li Xue Xian, Qiao Yuhui, Ding Guo-Chun, Li Ji
Beijing Key Laboratory of Biodiversity and Organic Farming, College of Resources and Environmental Sciences, China Agricultural University, Beijing, China.
Organic Recycling Institute (Suzhou) of China Agricultural University, Suzhou ViCheck Biotechnology Co., Ltd., Suzhou, China.
Front Microbiol. 2020 Aug 12;11:1861. doi: 10.3389/fmicb.2020.01861. eCollection 2020.
Organic greenhouse farming is an innovative system that may maintain a high yield and healthy agroecosystem. There have been no rigorous studies on the comparison of total and nitrogen-cycling bacterial community in vegetable soils between organic and conventional farming management at large scale. A survey of bacterial community and nitrogen cycles from soils under organic and conventional greenhouse farming was performed at 30 sites, covering seven soil types with 4 to 18 years of organic farming history. Communities of the total, diazotrophs and ammonia-oxidizing bacteria were studied with high-throughput sequencing of the 16S , and genes, respectively. Organic greenhouse farming did not influence alpha diversities. Beta diversities among the total (26/30) and diazotrophic (17/19) bacteria differed between farming systems, but compositional differences in ammonia-oxidizing bacteria between the two farming systems were only detected at 6 sites. Despite the effects of farming system on most bacterial genera were varied across different sites, organic greenhouse farming persistently selected for a few genera, possibly for the biodegradation of organic carbon with high molecular weight (, and ), but against putative ammonia oxidizing () and diazotrophic () bacterial genera, as determined by 16S analysis. Diazotrophic bacteria affiliated with cluster 1J were preferentially associated with organic greenhouse farming, in contrast to . In summary, this study provides insights into the complex effects of organic greenhouse farming on the total, diazotrophic and ammonia oxidizing bacterial communities across different environmental context.
有机温室种植是一种创新系统，可维持高产和健康的农业生态系统。目前尚未有大规模的严格研究来比较有机和传统种植管理方式下蔬菜土壤中总细菌群落和氮循环细菌群落。我们对30个地点的有机和传统温室种植土壤中的细菌群落和氮循环进行了调查，这些地点涵盖了7种土壤类型，有机种植历史为4至18年。分别通过对16S、 和 基因的高通量测序研究了总细菌、固氮菌和氨氧化细菌的群落。有机温室种植并未影响α多样性。总细菌（26/（此处原文有误，应为26/30））和固氮细菌（17/19）在不同种植系统间的β多样性存在差异，但仅在6个地点检测到两种种植系统间氨氧化细菌的组成差异。尽管种植系统对大多数细菌属的影响在不同地点有所不同，但有机温室种植持续选择了少数几个属，可能是为了对高分子量有机碳（如 和 ）进行生物降解，但不利于推测的氨氧化（ ）和固氮（ ）细菌属，这是通过16S 分析确定的。与 相比，隶属于1J簇的固氮细菌优先与有机温室种植相关。总之，本研究深入探讨了有机温室种植在不同环境背景下对总细菌、固氮细菌和氨氧化细菌群落的复杂影响。
