Li Wei, Li Hao, Zheng Shujuan, Wang Zewei, Sheng Huanjing, Shi Chunlei, Shi Xianming, Niu Qinya, Yang Baowei
College of Food Science and Engineering, Northwest A&F University, Yangling, China.
MOST-USDA Joint Research Center for Food Safety, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
Front Microbiol. 2020 Aug 4;11:1482. doi: 10.3389/fmicb.2020.01482. eCollection 2020.
Poultry products such as eggs provide essential nutrients to the human body and thus play vital roles in the human food network. is one of the most notorious foodborne pathogens and has been found to be prevalent in eggs. To better understand the characteristics of in eggs, we investigated the prevalence of spp. in 814 fresh eggs collected from poultry farms and retail marketplaces in Yangling, Shaanxi Province, China. The serotype, genotype, and antibiotic susceptibilities of 61 isolates recovered from the eggs were analyzed. The average detection rate of -positive eggs was 5.6%, with 6.6% of the eggs collected from poultry farms and 5.1% from marketplaces. Thirteen serotypes were identified from the 61 isolates, among which Typhimurium (24.5%) and Indiana (22.9%) were the most prevalent serotypes. Other dominant serotypes included Thompson (13.1%) and Enteritidis (11.4%), with the remaining nine serotypes detected at low rates (1.6-4.9%). All the isolates tested were resistant to sulfisoxazole (100.0%). The majority (77.1%) of the isolates were resistant to nalidixic acid, amoxicillin-clavulanate, and ampicillin, while nearly two-thirds (63.9-68.9%) were resistant to trimethoprim-sulfamethoxazole, kanamycin, tetracyclines, and chloramphenicol. The rate of resistance to ciprofloxacin was 40.1%; the resistance rates to streptomycin, ceftiofur, and ceftriaxone ranged from 21.3 to 26.2%; and those to gentamicin, amikacin, and cefoxitin were relatively low (3.3-16.4%). Forty-nine (80.3%) isolates exhibited resistance to multiple antibiotics, 20 (32.8%) of which were resistant to at least 10 antibiotics. Subtyping by pulse-field gel electrophoresis revealed a close genetic relatedness of isolates from poultry farms, in striking contrast to the high diversity of the isolates obtained from marketplaces. Isolates of the same serotype always shared identical genotype and antibiotic resistance profiles, even the ones that were recovered from eggs sampled at different locations and times. These findings indicate that diverse spp. with high rates of multidrug resistance are prevalent in fresh eggs in the study area. More attention should be paid to egg production, transportation, and storage to prevent foodborne outbreaks caused by .
鸡蛋等家禽产品为人体提供必需的营养物质，因此在人类食物网络中发挥着至关重要的作用。[病原体名称]是最臭名昭著的食源性病原体之一，已被发现普遍存在于鸡蛋中。为了更好地了解鸡蛋中[病原体名称]的特征，我们调查了从中国陕西省杨凌的家禽养殖场和零售市场收集的814枚新鲜鸡蛋中[病原体名称]属的流行情况。分析了从鸡蛋中分离出的61株[病原体名称]菌株的血清型、基因型和抗生素敏感性。[病原体名称]阳性鸡蛋的平均检出率为5.6%，其中从家禽养殖场收集的鸡蛋检出率为6.6%，从市场收集的鸡蛋检出率为5.1%。从61株分离物中鉴定出13种血清型，其中鼠伤寒[病原体名称]（24.5%）和印第安纳[病原体名称]（22.9%）是最常见的血清型。其他优势血清型包括汤普森[病原体名称]（13.1%）和肠炎[病原体名称]（11.4%），其余9种血清型检出率较低（1.6 - 4.9%）。所有测试的[病原体名称]分离物均对磺胺异恶唑耐药（100.0%）。大多数分离物（77.1%）对萘啶酸、阿莫西林 - 克拉维酸和氨苄西林耐药，而近三分之二（63.9 - 68.9%）对甲氧苄啶 - 磺胺甲恶唑、卡那霉素、四环素和氯霉素耐药。对环丙沙星的耐药率为40.1%；对链霉素、头孢噻呋和头孢曲松的耐药率在21.3%至26.2%之间；对庆大霉素、阿米卡星和头孢西丁的耐药率相对较低（3.3 - 16.4%）。49株（80.3%）[病原体名称]分离物表现出对多种抗生素耐药，其中20株（32.8%）对至少10种抗生素耐药。通过脉冲场凝胶电泳进行亚型分析显示，家禽养殖场分离的[病原体名称]菌株具有密切的遗传相关性，这与从市场获得的分离物的高度多样性形成鲜明对比。相同血清型的分离物总是具有相同的基因型和抗生素耐药谱，即使是从不同地点和时间采集的鸡蛋中分离得到的。这些发现表明，具有高多重耐药率的多种[病原体名称]属菌株在研究区域的新鲜鸡蛋中普遍存在。应更加关注鸡蛋的生产、运输和储存，以预防由[病原体名称]引起的食源性疾病暴发。
