Leiten Elise Orvedal, Nielsen Rune, Wiker Harald Gotten, Bakke Per Sigvald, Martinsen Einar Marius Hjellestad, Drengenes Christine, Tangedal Solveig, Husebø Gunnar Reksten, Eagan Tomas Mikal Lind
Dept of Clinical Science, University of Bergen, Bergen, Norway.
Dept of Thoracic Medicine, Haukeland University Hospital, Bergen, Norway.
ERJ Open Res. 2020 Aug 31;6(3). doi: 10.1183/23120541.00168-2020. eCollection 2020 Jul.
The aim of this study was to investigate whether the compositionality of the lower airway microbiota predicts later exacerbation risk in persons with COPD in a cohort study.
We collected lower airways microbiota samples by bronchoalveolar lavage and protected specimen brushes, and oral wash samples from 122 participants with COPD. Bacterial DNA was extracted from all samples, before we sequenced the V3-V4 region of the 16S RNA gene. The frequency of moderate and severe COPD exacerbations was surveyed in telephone interviews and in a follow-up visit. Compositional taxonomy and α and β diversity were compared between participants with and without later exacerbations.
The four most abundant phyla were Firmicutes, Bacteroidetes, Proteobacteria and Fusobacteria in both groups, and the four most abundant genera were , , and . The relative abundances of different taxa showed a large variation between samples and individuals, and no statistically significant difference of either compositional taxonomy, or α or β diversity could be found between participants with and without COPD exacerbations within follow-up.
The findings from the current study indicate that individual differences in the lower airway microbiota in persons with COPD far outweigh group differences between frequent and nonfrequent COPD exacerbators, and that the compositionality of the microbiota is so complex as to present large challenges for use as a biomarker of later exacerbations.
本研究旨在通过一项队列研究，调查下呼吸道微生物群的组成是否能预测慢性阻塞性肺疾病（COPD）患者日后的急性加重风险。
我们通过支气管肺泡灌洗和保护性标本刷收集了122例COPD患者的下呼吸道微生物群样本以及口腔冲洗样本。在对16S RNA基因的V3 - V4区域进行测序之前，从所有样本中提取细菌DNA。通过电话访谈和随访调查了中度和重度COPD急性加重的发生频率。比较了有和没有日后急性加重的参与者之间的组成分类以及α和β多样性。
两组中最丰富的四个门均为厚壁菌门、拟杆菌门、变形菌门和梭杆菌门，最丰富的四个属分别为 、 、 和 。不同分类群的相对丰度在样本和个体之间存在很大差异，在随访期间，有和没有COPD急性加重的参与者之间，无论是组成分类还是α或β多样性，均未发现统计学上的显著差异。
本研究结果表明，COPD患者下呼吸道微生物群的个体差异远远超过频繁和不频繁COPD急性加重者之间的组间差异，并且微生物群的组成非常复杂，以至于作为日后急性加重的生物标志物面临巨大挑战。
