金纳米簇作为一种对抗的抗菌替代物。

BACKGROUND: infection (CDI) has become one of the most important factors threatening human health, and about 20-30% antibiotic-related diarrhea cases and almost all pseudomembranous enteritis cases are related to CDI. The high recurrence of and the emergence of drug resistance make clinical treatment of CDI difficult. Therefore, there is an urgent need to develop a non-antibiotic-alternative therapy against CDI. Gold nanoclusters (AuNCs) can better interact with bacteria due to its ultrasmall size. The aim of the present study was to explore whether AuNCs could be used as an antibacterial agent to kill . METHODS: AuNCs and were co-cultivated in an anaerobic atmosphere to evaluate the bactericidal effect of AuNCs. The bacterial growth rate was estimated by using two concentrations of AuNCs (50 and 100 μM). The damage of AuNCs to is detected by SYTOX Green staining methods and SEM image analysis. The mechanism of AuNCs on was explored by reactive oxygen species (ROS) detection. The toxic effect of AuNCs on human cells was evaluated by MTT method. RESULTS: AuNCs (100 μM) killed drastically. AuNCs increased the release of ROS by about 5 fold and destroyed the membrane integrity of cells without causing significant toxic effect on human cells. CONCLUSION: AuNCs showed great potential as an alternative to traditional antibiotics in killing and may prove to be an alternative to treat CDI.

背景：感染（CDI）已成为威胁人类健康的最重要因素之一，约 20-30%的抗生素相关性腹泻病例和几乎所有伪膜性肠炎病例都与 CDI 有关。复发率高和耐药性的出现使得 CDI 的临床治疗变得困难。因此，迫切需要开发一种非抗生素替代疗法来治疗 CDI。金纳米簇（AuNCs）由于其超小尺寸，可以更好地与细菌相互作用。本研究旨在探讨 AuNCs 是否可以用作抗菌剂来杀死。

方法：在厌氧气氛中共同培养 AuNCs 和，以评估 AuNCs 的杀菌效果。使用两种浓度的 AuNCs（50 和 100 μM）来估计细菌的生长速度。通过 SYTOX Green 染色方法和 SEM 图像分析检测 AuNCs 对的损伤。通过检测活性氧（ROS）来探索 AuNCs 对的作用机制。通过 MTT 法评估 AuNCs 对人细胞的毒性作用。

结果：AuNCs（100 μM）可明显杀死。AuNCs 使 ROS 的释放增加了约 5 倍，并破坏了细胞的膜完整性，而对人细胞没有明显的毒性作用。

结论：AuNCs 在杀死方面显示出巨大的潜力，可以替代传统抗生素，可能被证明是治疗 CDI 的一种替代方法。