Csepregi Lucia, Ehling Roy A, Wagner Bastian, Reddy Sai T
Department of Biosystems Science and Engineering, ETH Zurich, 4058 Basel, Switzerland.
iScience. 2020 Sep 25;23(9):101519. doi: 10.1016/j.isci.2020.101519. Epub 2020 Sep 1.
Advances in reading, writing, and editing DNA are providing unprecedented insights into the complexity of immunological systems. This combination of systems and synthetic biology methods is enabling the quantitative and precise understanding of molecular recognition in adaptive immunity, thus providing a framework for reprogramming immune responses for translational medicine. In this review, we will highlight state-of-the-art methods such as immune repertoire sequencing, immunoinformatics, and immunogenomic engineering and their application toward adaptive immunity. We showcase novel and interdisciplinary approaches that have the promise of transforming the design and breadth of molecular and cellular immunotherapies.
在DNA读取、写入和编辑方面的进展，正为我们提供前所未有的视角来洞察免疫系统的复杂性。系统生物学和合成生物学方法的结合，使我们能够对适应性免疫中的分子识别进行定量且精确的理解，从而为转化医学中重新编程免疫反应提供了一个框架。在这篇综述中，我们将重点介绍诸如免疫组库测序、免疫信息学和免疫基因组工程等前沿方法及其在适应性免疫中的应用。我们展示了一些新颖的跨学科方法，这些方法有望改变分子和细胞免疫疗法的设计及应用范围。
