没有证据表明 Wnt 配体在果蝇中对平面细胞极性是必需的。
No Evidence that Wnt Ligands Are Required for Planar Cell Polarity in Drosophila.
机构信息
Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA.
Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA; Howard Hughes Medical Institute, Boston, MA 02115, USA.
出版信息
Cell Rep. 2020 Sep 8;32(10):108121. doi: 10.1016/j.celrep.2020.108121.
The frizzled (fz) and dishevelled (dsh) genes are highly conserved members of both the planar cell polarity (PCP) pathway and the Wnt signaling pathway. Given these dual functions, several studies have examined whether Wnt ligands provide a tissue-scale orientation cue for PCP establishment during development, and these studies have reached differing conclusions. Here, we re-examine this issue in the Drosophila melanogaster wing and notum using split-Gal4 co-expression analysis, multiplex somatic CRISPR, and double RNAi experiments. Pairwise loss-of-function experiments targeting wg together with other Wnt genes, via somatic CRISPR or RNAi, do not produce PCP defects in the wing or notum. In addition, somatic CRISPR against evi (aka wntless), which is required for the secretion of Wnt ligands, did not produce detectable PCP phenotypes. Altogether, our results do not support the hypothesis that Wnt ligands contribute to PCP signaling in the Drosophila wing or notum.
卷曲（fz）和蓬乱（dsh）基因是平面细胞极性（PCP）途径和 Wnt 信号通路中高度保守的成员。鉴于这两种功能，一些研究已经研究了 Wnt 配体是否为发育过程中 PCP 建立提供组织尺度的定向线索，这些研究得出了不同的结论。在这里，我们使用分裂 Gal4 共表达分析、多路体 CRISPR 和双 RNAi 实验，在果蝇翅膀和体壁中重新检查了这个问题。通过体壁 CRISPR 或 RNAi 靶向 wg 以及其他 Wnt 基因的成对功能丧失实验，在翅膀或体壁中不会产生 PCP 缺陷。此外，针对 evi（又名 wntless）的体壁 CRISPR，它是 Wnt 配体分泌所必需的，没有产生可检测到的 PCP 表型。总的来说，我们的结果不支持 Wnt 配体有助于果蝇翅膀或体壁中 PCP 信号的假设。
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