Biomedical Engineering Research Center, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand; Biomaterial Engineering for Medical and Health Research Unit, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand; International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS), Ibaraki, Japan.
3B's Research Group, I3Bs - Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark - Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, Portugal; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal.
Int J Pharm. 2020 Nov 15;589:119844. doi: 10.1016/j.ijpharm.2020.119844. Epub 2020 Sep 6.
The administration of a drug-loaded implantable hydrogel at the tumor site after surgical resection is a viable approach to prevent the local recurrence or metastasis. Dimyristoyl glycerophosphorylglycerol (DMPG)-based liposomes were developed for inducing the rapid gelation of silk fibroin (SF) and delivering an anticancer drug, curcumin. Curcumin was loaded in the liposomes and the stability of curcumin was enhanced. The gelation time of liposome-induced SF hydrogels ranged from 3 min to more than 6 h. The biological activity of liposome-SF hydrogels was evaluated in vitro using L929 fibroblasts and MDA-MB-231 breast cancer cells. The release of curcumin can inhibit the growth of cancer cells. Both cells cultured on the surface of the hydrogels loaded with curcumin displayed low cell survival due to the combination of low cell attachment and cytotoxicity of curcumin. Liposome-SF hydrogels show potential as a sealant administered at the tumor site to eliminate residual cancer cells after tumor removal.
在手术切除后将载药植入型水凝胶施用于肿瘤部位是预防局部复发或转移的可行方法。基于二肉豆蔻酰基甘油磷酸甘油酯（DMPG）的脂质体被开发用于诱导丝素纤维（SF）的快速胶凝并递送抗癌药物姜黄素。姜黄素被载入脂质体中，从而提高了姜黄素的稳定性。脂质体诱导的 SF 水凝胶的胶凝时间从 3 分钟到超过 6 小时不等。使用 L929 成纤维细胞和 MDA-MB-231 乳腺癌细胞在体外评估了载姜黄素脂质体-SF 水凝胶的生物学活性。姜黄素的释放可以抑制癌细胞的生长。由于姜黄素的低细胞附着和细胞毒性的结合，在载有姜黄素的水凝胶表面培养的两种细胞的存活率均较低。脂质体-SF 水凝胶作为一种密封剂在肿瘤部位给药，在肿瘤切除后消除残留的癌细胞具有潜在的应用前景。
