Kim Hyojin, Lim Chae Yeon, Lee Dan Bi, Seok Jong Hyeon, Kim Kyung Hyun, Chung Mi Sook
Department of Food and Nutrition, Duksung Women's University, Seoul 01369, Korea.
Department of Biotechnology and Bioinformatics, Korea University, Sejong 30019, Korea.
Viruses. 2020 Sep 7;12(9):997. doi: 10.3390/v12090997.
Norovirus is the leading cause of nonbacterial foodborne disease outbreaks. Human noroviruses (HuNoVs) bind to histo-blood group antigens as the host receptor for infection. In this study, the inhibitory effects of fucoidans from brown algae, (LJ), and sporophyll, were evaluated against murine norovirus (MNoV), feline calicivirus (FCV) and HuNoV. Pretreatment of MNoV or FCV with the fucoidans at 1 mg/mL showed high antiviral activities, with 1.1 average log reductions of viral titers in plaque assays. They also showed significant inhibition on the binding of the P domains of HuNoV GII.4 and GII.17 to A- or O-type saliva and the LJ fucoidan was the most effective, reaching 54-72% inhibition at 1 mg/mL. In STAT1 mice infected with MNoV, oral administration of the LJ fucoidan, composed of mainly sulfated fucose and minor amounts of glucose and galactose, improved the survival rates of mice and significantly reduced the viral titers in their feces. Overall, these results provide the LJ fucoidan can be used to reduce NoV outbreaks.
诺如病毒是细菌性食源性疾病暴发的主要原因。人诺如病毒（HuNoVs）作为感染的宿主受体与组织血型抗原结合。在本研究中，评估了来自褐藻、海带（LJ）和孢子叶的岩藻聚糖对鼠诺如病毒（MNoV）、猫杯状病毒（FCV）和HuNoV的抑制作用。用1mg/mL的岩藻聚糖预处理MNoV或FCV显示出高抗病毒活性，在空斑试验中病毒滴度平均降低1.1个对数。它们还对HuNoV GII.4和GII.17的P结构域与A或O型唾液的结合表现出显著抑制，并且LJ岩藻聚糖最有效，在1mg/mL时达到54-72%的抑制率。在感染MNoV的STAT1小鼠中，口服主要由硫酸化岩藻糖以及少量葡萄糖和半乳糖组成的LJ岩藻聚糖，提高了小鼠的存活率，并显著降低了它们粪便中的病毒滴度。总体而言，这些结果表明LJ岩藻聚糖可用于减少诺如病毒暴发。
