Sykes Arran, Saalbach Lisa, Benson Sam, Nestoros Eleni, Tobin Rachael, Yi Xin, Tanner Michael G, Vendrell Marc, Buller Gerald S
Institute of Photonics and Quantum Sciences, Heriot-Watt University, EH14 4AS Edinburgh, United Kingdom.
Centre for Inflammation Research, The University of Edinburgh, Edinburgh BioQuarter, 4-5 Little France Drive, EH16 4UU Edinburgh, United Kingdom.
Biomed Opt Express. 2025 Jul 7;16(8):3075-3083. doi: 10.1364/BOE.568337. eCollection 2025 Aug 1.
Singlet-oxygen, the first excited state of molecular oxygen (O), is a reactive oxygen species that plays a key role as a cytotoxic agent in photodynamic therapy (PDT). In this work, we report a highly light-sensitive detection system based on a single-photon avalanche diode (SPAD) detector and time-correlated single-photon counting (TCPSC) for real-time detection of luminescence signatures from photosensitized singlet-oxygen. Dynamics of singlet-oxygen produced by the excitation of small-scale organic nitrobenzoselenadiazole photosensitizers were extracted with acquisition times as short as 1 second. In a clinical setting, the ability to detect singlet-oxygen production in short time frames could allow for real-time adjustments in light dosing, ensuring sufficient singlet-oxygen production and complete treatment of diseased tissue in applications such as PDT.
单线态氧是分子氧（O）的第一激发态，是一种活性氧物质，在光动力疗法（PDT）中作为细胞毒性剂发挥关键作用。在这项工作中，我们报告了一种基于单光子雪崩二极管（SPAD）探测器和时间相关单光子计数（TCPSC）的高灵敏度检测系统，用于实时检测光敏单线态氧的发光特征。通过短至1秒的采集时间，提取了由小规模有机硝基苯并硒二唑光敏剂激发产生的单线态氧的动力学信息。在临床环境中，短时间内检测单线态氧产生的能力可以实现光剂量的实时调整，确保在PDT等应用中产生足够的单线态氧并完全治疗病变组织。
