对水牛乳腺上皮细胞乳脂合成的影响。

Effect of on the milk fat synthesis of buffalo mammary epithelial cells.

机构信息

Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, 650201, China.

Teaching Demonstration Center of the Basic Experiments of Agricultural Majors, Yunnan Agricultural University, Kunming, Yunnan, 650201, China.

出版信息

J Dairy Res. 2020 Aug;87(3):349-355. doi: 10.1017/S0022029920000710. Epub 2020 Sep 10.

DOI:10.1017/S0022029920000710

PMID:32907640

Abstract

We hypothesized that insulin-induced gene 1 (INSIG1) affects milk fat synthesis in buffalo. For this reason, the protein abundance of INSIG1 in the mammary tissue of buffalo during the peak period of lactation and dry-off period was evaluated. The results showed that the expression of INSIG1 at the peak of lactation was lower than that in the dry-off period. To explore the role of INSIG1 in milk fat synthesis, the buffalo mammary epithelial cells (BMECs) were isolated and purified from buffalo mammary tissue, and INSIG1 gene were overexpressed and knocked down by constructing the recombinant lentivirus vector of INSIG1 gene and transfecting into BMECs. Results revealed that INSIG1 overexpression decreased the expression of INSIG2, SREBP, PPARG, SCD, GPAM, DGAT2 and AGPAT6, which led to reduction of triglycerides (TAG) content in the cell. In contrast, knockdown of INSIG1 had a positive effect on mRNA expression of the above genes. Overall, the data provide strong support for a key role of INSIG1 in the regulation of milk fat synthesis in BMECs.

摘要

我们假设胰岛素诱导基因 1（INSIG1）会影响水牛的乳脂合成。出于这个原因，评估了泌乳高峰期和干奶期水牛乳腺组织中 INSIG1 的蛋白丰度。结果表明，泌乳高峰期 INSIG1 的表达低于干奶期。为了探究 INSIG1 在乳脂合成中的作用，我们从水牛乳腺组织中分离和纯化了水牛乳腺上皮细胞（BMECs），并通过构建 INSIG1 基因的重组慢病毒载体转染到 BMECs 中，实现了 INSIG1 基因的过表达和敲低。结果表明，INSIG1 过表达降低了 INSIG2、SREBP、PPARG、SCD、GPAM、DGAT2 和 AGPAT6 的表达，导致细胞内甘油三酯（TAG）含量减少。相比之下，敲低 INSIG1 对上述基因的 mRNA 表达有积极影响。总体而言，这些数据为 INSIG1 在 BMECs 乳脂合成调节中的关键作用提供了有力支持。