Makino Ryosuke, Sugahara Misaki, Kita Kazumi
The United Graduate School of Agricultural Sciences, Iwate University, Morikoka, Iwate 020-8550, Japan.
Laboratory of Animal Nutrition, Faculty of Agriculture, Iwate University, Morikoka, Iwate 020-8550, Japan.
J Poult Sci. 2016 Jul 25;53(3):220-222. doi: 10.2141/jpsa.0150112.
Avian species including chickens are known to be hyperglycemic animals. Hyperglycemia promotes the glycation which at first forms Amadori products undergoing further complex reaction to form advanced glycation end products (AGEs). Our previous study revealed that AGEs derived from glucose and amino acids were predominantly incorporated into spleen, kidney and liver. However, it has not been elucidated whether Amadori products (glycated amino acids) can also be incorporated into cells or not. Therefore, in the present study, radioactive glycated-tryptophan and -valine were prepared and the incorporation of these glycated amino acids into various chicken embryonic cells was studied. Various embryonic cells prepared from muscle, liver, spleen and kidney of chicken embryos were incubated in Medium 199 supplemented with C-labeled glycated-tryptophan or -valine. After incubation, embryonic cells were well-rinsed and then the radioactivity incorporated into cells was measured. It was revealed that both glycated amino acids were incorporated into embryonic cells derived from muscle, liver, spleen and kidney. In muscular cells, the incorporation of glycated-tryptophan was higher than that of glycated-valine. On the other hand, in embryonic cells derived from liver and kidney, the amount of glycated-tryptophan incorporated into cells was almost the same to that of glycated-valine. In conclusion, it was supposed that both glycated-tryptophan and -valine could be incorporated into various cells derived from muscle, liver, spleen and kidney of chicken embryos and that the incorporation might have the organ specificity.
包括鸡在内的禽类是已知的高血糖动物。高血糖会促进糖基化反应，首先形成阿马多里产物，该产物会进一步发生复杂反应形成晚期糖基化终产物（AGEs）。我们之前的研究表明，源自葡萄糖和氨基酸的AGEs主要会被整合到脾脏、肾脏和肝脏中。然而，阿马多里产物（糖化氨基酸）是否也能被整合到细胞中尚未得到阐明。因此，在本研究中，制备了放射性糖化色氨酸和缬氨酸，并研究了这些糖化氨基酸在各种鸡胚细胞中的整合情况。将从鸡胚的肌肉、肝脏、脾脏和肾脏制备的各种胚细胞在补充有C标记的糖化色氨酸或缬氨酸的199培养基中孵育。孵育后，将胚细胞充分冲洗，然后测量整合到细胞中的放射性。结果表明，两种糖化氨基酸都能被整合到源自肌肉、肝脏、脾脏和肾脏的胚细胞中。在肌肉细胞中，糖化色氨酸的整合量高于糖化缬氨酸。另一方面，在源自肝脏和肾脏的胚细胞中，整合到细胞中的糖化色氨酸量与糖化缬氨酸量几乎相同。总之，推测糖化色氨酸和缬氨酸都能被整合到鸡胚的肌肉、肝脏、脾脏和肾脏的各种细胞中，并且这种整合可能具有器官特异性。
