Krasniqi Berat, Thaçi Shpëtim, Dërmaku-Sopjani Miribane, Rifati-Nixha Arleta, Abazi Sokol, Sopjani Mentor
Faculty of Medicine, University of Prishtina, Prishtina 10 000, Kosovo.
Faculty of Natural Sciences and Mathematics, University of Prishtina, Prishtina 10 000, Kosovo.
Evid Based Complement Alternat Med. 2020 Aug 28;2020:6510708. doi: 10.1155/2020/6510708. eCollection 2020.
Boiss. and Heldr. (Lamiaceae), known as "mountain tea," is a native plant from the Mediterranean region, which is widely used in traditional medicine. This study evaluates the effects of the ethanol extract of (SR) on airway smooth muscle activity and identifies the underlying mechanism. The extract (SRE) was extracted from air-dried parts of the shoot system of SR. The SRE (0.3-2 mg/mL) was tested in isolated rabbit tracheal rings, suspended in the organ bath, filled with Krebs solution, and bubbled with the carbogen mixture (95% O/5% CO) under a resting tension of 1 g in 37°C. In experiments, the SRE relaxed against acetylcholine-induced constriction in tracheal rings. Furthermore, SRE inhibited Ca-induced contractions in carbachol (CCh, 1 M) as well as in the K-depolarized trachea (80 mM). Our findings showed the NO/cGMP involvement in tracheorelaxant effects of SR. To this end, the effect of the SRE was potentiated by bradykinin (nitric oxide (NO) synthase activator, 100 nM), whereas it was inhibited by ODQ (inhibitor of NO-sensitive guanylyl cyclase, 10 M) and L-NAME (NO synthase inhibitor, 100 M), as well as indomethacin (cyclooxygenase inhibitor, 10 M). These data suggest that the tracheorelaxant effect of the SRE is mediated at least partly by NO/cyclic guanosine monophosphate and cyclooxygenase-1-prostaglandin E2-dependent signaling. These findings indicate that the SRE may be used in various respiratory disorders.
博伊斯和赫尔德（唇形科），被称为“山茶”，是一种原产于地中海地区的本土植物，在传统医学中被广泛使用。本研究评估了（SR）乙醇提取物对气道平滑肌活动的影响，并确定其潜在机制。SR提取物（SRE）是从SR地上部分干燥的枝条中提取的。将SRE（0.3 - 2毫克/毫升）在离体兔气管环中进行测试，气管环悬浮于器官浴中，浴中充满克雷布斯溶液，并在37℃下1克的静息张力下用混合气体（95%氧气/5%二氧化碳）鼓泡。在实验中，SRE可舒张气管环中乙酰胆碱诱导的收缩。此外，SRE可抑制卡巴胆碱（CCh，1微摩尔）以及钾去极化气管（80毫摩尔）中钙诱导的收缩。我们的研究结果表明，一氧化氮/环磷酸鸟苷参与了SR的气管舒张作用。为此，缓激肽（一氧化氮合酶激活剂，100纳摩尔）可增强SRE的作用，而ODQ（一氧化氮敏感鸟苷酸环化酶抑制剂，10微摩尔）、L - 精氨酸甲酯（一氧化氮合酶抑制剂，100微摩尔）以及吲哚美辛（环氧化酶抑制剂，10微摩尔）则可抑制其作用。这些数据表明，SRE的气管舒张作用至少部分是由一氧化氮/环磷酸鸟苷和环氧化酶 - 1 - 前列腺素E2依赖性信号介导的。这些研究结果表明，SRE可用于治疗各种呼吸系统疾病。
