Department of Physiology, Jining Medical College, Jining, Shandong, China.
Department of Pharmacy, Jining Medical College, Jining, Shandong, China.
Comput Math Methods Med. 2020 Aug 25;2020:3128053. doi: 10.1155/2020/3128053. eCollection 2020.
Coronary heart disease (CHD) is the most common cardiovascular disease with high prevalence, disability, and mortality. The balance between proliferation and apoptosis of vascular smooth muscle cells (VSMCs) plays a key role in the initiation of atherosclerosis. In this study, we found a significant decrease in the expression of lncRNA-SNHG14 in atherosclerotic plaque tissues of ApoE-/- mice. Overexpression of lncRNA-SNHG14 can inhibit VSMC proliferation while promoting apoptosis. There is a potential reciprocal regulatory relationship between lncRNASNHG14 and miR-19a-3p, which inhibit each other's expression in vascular smooth muscle cells. In addition, the luciferase reporter gene analysis results showed that there was a direct interaction between miR-19a-3p and the 3'UTR of ROR. The results of qRT-PCR showed that the level of ROR mRNA was significantly increased in the aortas treated with miR-19a-3p and SNHG14 compared with that treated with miR-19a-3p alone. In conclusion, we demonstrated that lncRNA-SNHG14 regulates the apoptosis/proliferation balance of VSMCs in atherosclerosis.
冠心病（CHD）是最常见的心血管疾病，具有高患病率、高致残率和高死亡率。血管平滑肌细胞（VSMCs）的增殖和凋亡平衡在动脉粥样硬化的发生中起着关键作用。在本研究中，我们发现载脂蛋白 E 基因敲除（ApoE-/-）小鼠动脉粥样硬化斑块组织中 lncRNA-SNHG14 的表达显著降低。lncRNA-SNHG14 的过表达可以抑制 VSMC 的增殖，同时促进其凋亡。lncRNA-SNHG14 和 miR-19a-3p 之间存在潜在的相互调节关系，它们在血管平滑肌细胞中相互抑制表达。此外，荧光素酶报告基因分析结果表明 miR-19a-3p 与 ROR 的 3'UTR 之间存在直接相互作用。qRT-PCR 结果显示，与单独转染 miR-19a-3p 相比，转染 miR-19a-3p 和 SNHG14 可使主动脉中 ROR mRNA 的水平显著升高。综上所述，我们证明了 lncRNA-SNHG14 调节动脉粥样硬化中 VSMCs 的凋亡/增殖平衡。
